Sökning: "Candida antarctica Lipase A"

Visar resultat 11 - 15 av 58 avhandlingar innehållade orden Candida antarctica Lipase A.

  1. 11. Expression and Mutagenesis studies of Candida antactica lipase B

    Författare :Johanna C. Rotticci-Mulder; KTH; []
    Nyckelord :Protein expression; Candida antarctica lipase B; Pichia pastoris; site-directed mutagenesis; fermentation; selection;

    Sammanfattning : Recombinant Candida antarctica lipase B was successfullyproduced in the methylotropic yeast Pichia pastoris. Thespecific activities of Candida antarctica lipase B produced inPichia pastoris and commercial Candida antarctica lipase B fromNovozymes were the same. In shake-flask cultivations theexpression levels were about 25 mg L-1. LÄS MER

  3. 12. Enzyme substrate solvent interactions : a case study on serine hydrolases

    Författare :Linda Fransson; Karl Hult; Jürgen Pleiss; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; lipase; esterase; specificity; Bioengineering; Bioteknik;

    Sammanfattning : Reaction rates and selectivities were measured for transacylation of fatty acid esters in solvents catalysed by Candida antarctica lipase B and by cutinase from Humicola insolens. With these enzymes classical water-based enzymology can be expanded to many different solvents allowing large variations in interaction energies between the enzymes, the substrates and the surrounding. LÄS MER

  4. 13. Synthetic Transformations via Metal- and Enzyme-Catalyzed Dynamic Kinetic Resolution

    Författare :Krisztián Bogár; Jan-Erling Bäckvall; Romas Kazlauskas; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; ruthenium; racemization; lipase; dynamic kinetic resolution; secondary alcohol; Candida antarctica lipase B; Organic chemistry; Organisk kemi; Organic Chemistry; organisk kemi;

    Sammanfattning : This thesis deals with the preparation of a new half-sandwich type ruthenium(II)- catalyst for racemization of optically active secondary alcohols and the development of a highly efficient method in combination with lipases such as Candida antarctica lipase B and Pseudomonas cepacia lipase for dynamic kinetic resolution of various functionalized alcohols under mild reaction conditions.It was shown that the RuCl(CO)2(η5-C5Ph5) complex can racemize optically active aliphatic and aromatic secondary alcohols at room temperature in rather short times. LÄS MER

  5. 14. Lipase-Catalysed Lipid Modifications in Supercritical Carbon Dioxide

    Författare :Helga Gunnlaugsdottir; Livsmedelsteknik; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; immobilisation support; enzymes; pressure; phase behaviour; flow rate; process parameters; supercritical carbon dioxide; Lipase; lipids; Food and drink technology; Livsmedelsteknik;

    Sammanfattning : The production of ethyl esters (EE) from ethanol and cod-liver oil by an immobilised lipase in supercritical carbon dioxide (SCCO2) is described. This reaction results in a complex mixture of lipid components consisting of triglycerides, diglycerides, monoglycerides and ethyl esters. LÄS MER

  7. 15. Structural Determination and Directed Evolution of Candida antarctica Lipase A for Increased Enantioselectivity

    Författare :Anders Sandström; Jan-Erling Bäckvall; Eszter Borbas; Stockholms universitet; []
    Nyckelord :;

    Sammanfattning : .... LÄS MER