Avancerad sökning

Visar resultat 1 - 5 av 58 avhandlingar som matchar ovanstående sökkriterier.

  1. 1. Protein Engineering of Candida antarctica Lipase A : Enhancing Enzyme Properties by Evolutionary and Semi-Rational Methods

    Författare :Anders G. Sandström; Jan-Erling Bäckvall; Uwe T. Bornscheuer; Stockholms universitet; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; lipase; protein engineering; directed evolution; kinetic resolution; structural biology; Enzyme engineering; Enzymteknik; organisk kemi; Organic Chemistry;

    Sammanfattning : Enzymes are gaining increasing importance as catalysts for selective transformations in organic synthetic chemistry. The engineering and design of enzymes is a developing, growing research field that is employed in biocatalysis. LÄS MER

  3. 2. Rational redesign of Candida antarctica lipase B

    Författare :Anders Magnusson; Karl Hult; Bernhard Hauer; KTH; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Biochemistry; Candida antarctica lipase B; rational redesign; secondary alcohols; substrate-assisted catalysis; S-selective; entropy; aldolase; stereospecificity pocket; oxyanion hole.; Biokemi; Biochemistry; Biokemi;

    Sammanfattning : This thesis describes the use of rational redesign to modify the properties of the enzyme Candida antarctica lipase B. Through carefully selected single-point mutations, we were able to introduce substrate-assisted catalysis and to alter the reaction specificity. LÄS MER

  4. 3. Engineering Candida antarctica Lipase A for Enantioselective Transformations in Organic Synthesis : Design, Immobilization and Organic Solvent Screening of Smart Enzyme Libraries

    Författare :Ylva Wikmark; Jan-Erling Bäckvall; Romas Kazlauskas; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Candida antarctica Lipase A; protein engineering; enzyme libraries; enzyme immobilization; biocatalysis; organisk kemi; Organic Chemistry;

    Sammanfattning : The use of enzymes as catalysts in organic synthesis constitutes an attractive alternative to conventional chemical catalysis. Enzymes are non-toxic and biodegradable and they can operate under mild reaction conditions. LÄS MER

  5. 4. Enthalpy and Entropy in Enzyme Catalysis : A Study of Lipase Enantioselectivity

    Författare :Jenny Ottosson; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Biocatalysis; enzyme catalysis; Candida antarctica lipase B; enantioselectivity; enthalpy; entropy; CALB; enantiomeric ratio; Bioengineering; Bioteknik;

    Sammanfattning : Biocatalysis has become a popular technique in organic synthesis due to high activity and selectivity of enzyme catalyzed reactions. Enantioselectivity is a particularly attractive enzyme property, which is utilized for the production of enantiopure substances. LÄS MER

  7. 5. Engineering of Candida antarctica Lipase A and B

    Författare :Johanna Löfgren; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES;

    Sammanfattning : In organic chemistry, it is often desirable to have an efficient process yieldingpure product and not too much waste. When applicable, it is also desirable tohave a high enantioselective outcome. Enzymes are often displaying high regio-,chemo- and stereoselectivity. LÄS MER