Sökning: "Caco-2"

Visar resultat 1 - 5 av 63 avhandlingar innehållade ordet Caco-2.

1. 1. Refined in vitro Models for Prediction of Intestinal Drug Transport : Role of pH and Extracellular Additives in the Caco-2 Cell Model

   Författare :Sibylle Neuhoff; Per Artursson; Anna-Lena Ungell; Patrick Augustijns; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Pharmacy; Caco-2 cells; membrane permeability; drug permeation; drug efflux ratios; drug uptake ratios; Cremophor EL; bovine serum albumin; pH-dependent permeability; drug transport; FARMACI; PHARMACY; FARMACI;

   Sammanfattning : Drug transport across the intestinal epithelium is roughly predicted from permeability values obtained from Caco-2 cell monolayers. This thesis examines the important role of pH and extracellular additives for increasing the reliability and predictivity of the in vitro screening system, Caco-2. LÄS MER

3. 2. Oxidation of marine oils during in vitro gastrointestinal digestion and its effects on stress in human intestinal Caco-2 cells

   Författare :Cecilia Tullberg; Chalmers tekniska högskola; []
   Nyckelord :LANTBRUKSVETENSKAPER; AGRICULTURAL SCIENCES; lipid oxidation; human digestive fluids; LC n-3 PUFAs; Caco-2 cells; marine oils; HNE; MDA; in vitro digestion; HHE; gastrointestinal; aldehydes;

   Sammanfattning : Marine oils are attracting public interest due to the preventive effects, e.g., on inflammation, which are linked to the long-chain n-3 polyunsaturated fatty acids (LC n-3 PUFAs). However, LC n-3 PUFAs are highly susceptible to oxidation, which could interfere with their positive effects. LÄS MER

4. 3. Proteomic and Functional Analysis of In Vitro Systems for Studies of Drug Disposition in the Human Small Intestine and Liver

   Författare :Magnus Ölander; Per Artursson; Pär Matsson; Edward L. LeCluyse; Volker Lauschke; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; proteomics; drug disposition; ADMET; drug transport; drug metabolism; hepatotoxicity; small intestine; liver; caco-2; human hepatocytes; cryopreservation; apoptosis; liver zonation; non-parenchymal cells; Farmaceutisk vetenskap; Pharmaceutical Science;

   Sammanfattning : To reach the bloodstream, an orally administered drug must be absorbed through the small intestine and avoid extensive clearance in the liver. Estimating these parameters in vitro is therefore important in drug discovery and development. LÄS MER

5. 4. Bioactive Food Compounds with Cancer Preventing Effects in the Colon Focus on Ferulic Acid, para-Coumaric Acid and Lactoferricin

   Författare :Birgit Janicke; Livsmedelsteknik; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Caco-2; Gene expression; Cell proliferation; Cell cycle; Bioactive food compounds; Colon cancer; Ferulic acid; para-Coumaric acid; Lactoferricin;

   Sammanfattning : Colorectal cancers are globally ranked as the third most common cancer form in terms of incidence and mortality. Diet has long been considered as one of the main factors affecting population outcome regarding development of cancer in the colon. LÄS MER

7. 5. Intestinal Permeability and Presystemic Extraction of Fexofenadine and R/S-verapamil

   Författare :Christer Tannergren; Hans Lennernäs; Dirk KF Meijer; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Biopharmacy; Bioavailability; Fexofenadine; Verapamil; P-glycoprotein; CYP3A4; OATP; Enantioselective; Permeability; Caco-2; Intestinal perfusion; Biofarmaci; Biopharmacy; Biofarmaci;

   Sammanfattning : The main objective of this thesis was to investigate the in vivo relevance of membrane transporters and cytochrome P450 (CYP) 3A4-mediated metabolism in the intestine and liver for the bioavailability of drugs in humans after oral administration.In the first part of the thesis, the main transport mechanisms involved in the intestinal absorption and bioavailability were investigated for fexofenadine, a minimally metabolized drug, which is a substrate for P-glycoprotein (P-gp) and members of organic anion transporting polypeptide (OATP) family. LÄS MER