Sökning: "CRISPR Cas9"

Visar resultat 16 - 20 av 78 avhandlingar innehållade orden CRISPR Cas9.

1. 16. Glycosaminoglycan Biosynthesis in Zebrafish

   Författare :Beata Filipek-Górniok; Lena Kjellén; Johan Ledin; Kay Grobe; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Heparan sulfate; chondroitin dermatan sulfate; biosynthesis; development; N-deacetylase N-sulfotransferase; glycosyltransferases; morpholino; CRISPR-Cas9;

   Sammanfattning : Proteoglycans (PGs) are composed of highly sulfated glycosaminoglycans chains (GAGs) attached to specific core proteins. They are present in extracellular matrices, on the cell surface and in storage granules of hematopoietic cells. LÄS MER

3. 17. Generation of human astrocytes for disease modeling. A study based on stem cells, direct conversion and genome engineering to dissect the role of astrocytes in leukodystrophies

   Författare :Ella Quist; Lund Neurologi; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Astrocytes; Stem Cells; transcription factor programming; direct conversion; genome engineering; CRISPR Cas9; leukodystrophy;

   Sammanfattning : Astrocytes are one of the major cell types in the central nervous system and are indispensable for brain development and function. The human brain and human astrocytes have unique structures and functions that are not present in other animal species. LÄS MER

4. 18. Precision mapping of gene expression and proteins in the brain using gene editing and barcoded viral vectors

   Författare :Martino Avallone; Molekylär neuromodulering; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Gene editing; CRISPR Cas9; Barcodes; AAV; PLA; gene therapy; Parkinson’s disease; single nuclei RNAseq; alpha-synuclein;

   Sammanfattning : The human brain is a masterpiece of intricate design and impeccable functionality. It serves as the ultimate command center for our thoughts, sensations, and actions, which define our very existence. LÄS MER

5. 19. Musculoskeletal Development in Jawed Vertebrates : Gene function, cis-regulation, and 3D phenotypes in zebrafish

   Författare :Jake Leyhr; Tatjana Haitina; Sophie Sanchez; Per Ahlberg; Chrissy Hammond; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Nkx3.2; Gdf5; Mkx; zebrafish; jaw joint; skeleton; fin development; cartilage; tendon; ligament; enhancer; CRISPR Cas9; mutant; microCT; synchrotron; Biology with specialization in Evolutionary Organismal Biology; Biologi med inriktning mot evolutionär organismbiologi;

   Sammanfattning : Vertebrate skeletons are an intricate framework of bony and cartilaginous structures that form through carefully orchestrated developmental processes, guided by interacting genetic pathways that regulate cellular differentiation, migration, and tissue morphogenesis. The specific timing and localisation of gene expression shapes the diverse array of skeletal elements, from the flexible cartilages of the embryonic stage to the hardened bones that provide structural support in adulthood, and the joints and connective tissues that articulate the musculoskeletal system. LÄS MER

7. 20. Mass spectrometric analyses of proteoglycans - Novel tools for studying prohormones in insulin-producing cells

   Författare :Mahnaz Nikpour; Göteborgs universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; proteoglycan; glycosaminoglycan; mass spectrometry; secretory granule; prohormones; CRISPR Cas9;

   Sammanfattning : Proteoglycans (PGs) are proteins that carry one or more negatively charged glycosaminoglycan (GAG) chains. Proteoglycans have been identified in essentially all multicellular organisms, and are implicated in a wide range of biological and pathological processes. LÄS MER