Sökning: "COS-7 cells"

Visar resultat 1 - 5 av 11 avhandlingar innehållade orden COS-7 cells.

1. 1. Molecular regulation of rat histidine decarboxylase

   Författare :Christine Dartsch; Institutionen för experimentell medicinsk vetenskap; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Physiology; omeprazole; antiserum; fetal rat liver; COS-7 cells; purification; bacterial expression; protein turnover; ECL cells; gastric mucosa; gastrin; histdine decarboxylase; histamine; Fysiologi; Proteins; enzymology; Proteiner; enzymologi;

   Sammanfattning : Histamine is involved in a variety of physiological processes like gastric acid secretion, inflammation and neurotransmission. The production of histamine is catalysed by the enzyme histidine decarboxylase (HDC), which is a strongly regulated enzyme. LÄS MER

3. 2. Expression of recombinant neutralizing anti-HIV-1 antibodies in bacteria and eukaryotic cells

   Författare :Fayezeh Yari; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :Phage display; vector; monoclonal antibody; antigen; eukaryotic; prokaryotic; transfection; selection; transient expression; stable cell line; neutralizing antibody; expression; HIVA-1; gp41; gp120;

   Sammanfattning : The main purpose of this study was to investigate and optimize techniques for eukaryotic expression of antibody molecules (IgG, Fab and scFV-ê formats), derived from an immune phage displayed Fab library and reactive against HIV-1 glycoproteins gp120 and gp41. Light and heavy-chain (Fd) DNA from a number of Fab clones were transferred from a prokaryotic phagemid vectors to mammalian expression vectors. LÄS MER

4. 3. Molecular and cellular mechanisms during adherence and cell signaling of pathogenic Neisseria to host cells

   Författare :Helena Källström; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :Neisseria; pili; adhesion; PiIC; CD46; signaling; G-protein;

   Sammanfattning : The pathogenic members of the genus Neisseria include N. gonorrhoeae and N. meningitidis. Both organisms are obligate human pathogens that colonize mucosal surfaces. LÄS MER

5. 4. On the Biosynthesis and Processing of Cathepsin G, Leukocyte Elastase, and Azurocidin ­ neutrophil granule members of a hematopoietic serine protease superfamily

   Författare :Anders Lindmark; Avdelningen för hematologi och transfusionsmedicin; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; extracellular fluids; Haematology; sorting; Activation; propeptide; Hematologi; extracellulära vätskor;

   Sammanfattning : The serine proteases cathepsin G, leukocyte elastase, proteinase 3, and the inactive protease homologue azurocidin, stored in the azurophil granules of neutrophils, belong to a hematopoietic serine protease superfamily with members in mature effector cells of bone marrow origin. Promonocytic U-937 cells were used for studying the biosynthesis of cathepsin G and leukocyte elastase by biosynthetic radiolabelling. LÄS MER

7. 5. Rational design of nanoparticles for biomedical imaging and photovoltaic applications

   Författare :Haiyan Qin; Hans Ågren; Alexander Dmitriev; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Theoretical chemistry; Teoretisk kemi;

   Sammanfattning : This thesis aims to rationally design nanoparticles and promote their applications in biomedical imaging and photovoltaic cells. Quantum dots (QDs) are promising fluorescent probes for biomedical imaging. LÄS MER