Sökning: "CHO cells"

Visar resultat 16 - 20 av 51 avhandlingar innehållade orden CHO cells.

  1. 16. High cell density perfusion process development for antibody producing Chinese Hamster Ovary cells

    Författare :Ye Zhang; Vincent Bulone; Veronique Chotteau; Carl-Fredrik Mandenius; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; WAVE Bioreactor; Cellbag; ATF; TFF; hollow fiber; CSPR; CellTank; SUB; single-use-bioreactor; disposable; perfusion rate; Biotechnology; Bioteknologi;

    Sammanfattning : Perfusion operation mode is currently under fast expansion in mammalian cell based manufacturing of biopharmaceuticals, not only for labile drug protein but also for stable proteins such as monoclonal antibodies (mAbs). Perfusion mode can advantageously offer a stable cell environment, long-term production with high productivity and consistent product quality. LÄS MER

  3. 17. Studies of Vibrio cholerae toxins (zonula occludens toxin and hemagglutinin/protease) and their effects on epithelial cells

    Författare :Zhengyang Wu; Åsa Ljung; Linköpings universitet; []
    Nyckelord :MEDICINE; MEDICIN;

    Sammanfattning : Intestinal epithelium is important for absorption of nutrients and for defense against harmful substances in the gut. The permeability and barrier functions of epithelia are mainly controlled by intercellular tight junctions. LÄS MER

  4. 18. Molecular and functional studies of interleukin-1a in the rat testis

    Författare :Taranum Sultana; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :Interleukin-l alpha; spermatogenesis; Leydig cells; Sertoli cells; calpain I and II;

    Sammanfattning : The aim of the present study was to gain a better insight into the role of interleukin-1 alpha (IL-1 alpha) in testicular physiology. In testis, IL-1alpha acts as a paracrine mediator involved in the regulation of testicular functions. LÄS MER

  5. 19. Polyamines are involved in the regulation of S phase and DNA synthesis

    Författare :Kersti Alm; Biologiska institutionen; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Djurfysiologi; Animal physiology; cell cycle regulation; p53; DNA strand breaks; replicon organization; topoisomerase II;

    Sammanfattning : Our research group has previously shown that treatment with the polyamine biosynthesis inhibitors a-difluoromethylornithine (DFMO) or amidinoindan-1-one 2´-amidinohydrazone (CGP 48664) inhibited S phase progression before any other cell cycle phase was affected. This study was undertaken to further investigate the role of polyamines in the regulation of S phase progression and DNA synthesis. LÄS MER

  7. 20. Humoral immunity during primate lentivirus infection : evaluation of B cell apoptosis and engineering of virus specific antibodies

    Författare :Astrid Samuelsson; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : The focus of this project was to evaluate the contribution of the humoral immunity in controlling infection with primate lentiviruses - an area which has implications both for vaccine design as well as for understanding of the pathogenesis of the disease. The first goal was to generate efficient neutralizing reagents which could be used for passive immunotherapy trials, preferably in the macaque system, and to investigate if peptide immunogens could elicit such antibodies. LÄS MER