Sökning: "Blue copper protein"
Visar resultat 6 - 10 av 13 avhandlingar innehållade orden Blue copper protein.
6. The use of protein biomarkers in ecotoxicology : Studies of oxidative and genotoxic stress in the blue mussel (Mytilus edulis)
Sammanfattning : Many environmental pollutants, heavy metals and polyaromatic hydrocarbons (PAHs) among them, are toxic by oxidative stress. Oxidative stress may be defined as a state in an organism when its inherent capacity to handle oxyradicals is surpassed, and it may result in peroxidation of lipids, and damage to proteins and DNA. LÄS MER
7. Protein Engineering on Azurin. Expression Mutagenesis and Characterisation of Copper Site Mutants
Sammanfattning : Azurin belongs to a family of small blue copper proteins or cupredoxins which participate in electron transfer processes in plants and bacteria. The type 1 copper site in these proteins is characterised by an intense blue colour, a narrow hyperfine coupling in the EPR signal and, generally, a high reduction potential. LÄS MER
8. Protein Expression in Baltic Sea Blue Mussels Exposed to Natural and Anthropogenic Stress : The use of stress inducible proteins in ecotoxicological studies
Sammanfattning : The focus of this thesis is the early detection of stress in the environment. It has been proposed that studies on the cellular level would detect stress reactions earlier in time compared to common physiological methods. LÄS MER
9. Theoretical studies of porphyrin proteins
Sammanfattning : Different aspects of porphyrin proteins have been studied with theoretical methods. For example, we have studied: - The importance of hydrogen bonds for the discrimination between carbon monoxide and molecular oxygen by myoglobin, the oxygen carrier in muscles, modelling the active site both in vacuum and in the protein matrix - The inner-sphere reorganisation energy during electron transfer for cytochromes compared to other electron-transfer proteins, such as blue copper proteins and Fe-S clusters - The role of porphyrin distortions in the reaction mechanism of ferrochelatase, the enzyme that inserts an iron ion into protoporphyrin IX to make haem - The interaction between different metal sites in ferrochelatase In these studies we have used density functional theory, classical force field methods, and a combination of quantum chemistry and molecular mechanics. LÄS MER
10. Spectroelectrochemistry of Redox Enzymes
Sammanfattning : A low volume and long optical path spectroelectrochemical cell was constructed and tested in a number of applications such as detection of direct electron transfer (DET) between redox enzymes and gold electrodes, determination of the formal potentials of redox centres in proteins and enzymes, as well as mechanistic studies of heterogeneous and intermolecular electron transfer of copper oxidases and succinate:quinine oxidoreductase (Complex II). The existence of DET between enzymes and electrodes was demonstrated for theophylline oxidase, cytochrome P450 cam, laccase, bilirubin oxidase, sulphite oxidase and Complex II. LÄS MER