Sökning: "Bioprocessteknik"

Visar resultat 1 - 5 av 61 avhandlingar innehållade ordet Bioprocessteknik.

  1. 1. Influence of recombinant passenger properties and process conditions on surface expression using the AIDA-I autotransporter

    Författare :Martin Gustavsson; Gen Larsson; Peter Neubauer; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; AIDA-I; Autotransport; Biocatalysis; Escherichia coli; Live vaccines; Surface expression;

    Sammanfattning : Surface expression has attracted much recent interest, and it has been suggested for a variety of applications. Two such applications are whole-cell biocatalysis and the creation of live vaccines. LÄS MER

  3. 2. Surface expression using the AIDA autotransporter : Towards live vaccines and whole-cell biocatalysis

    Författare :Martin Gustavsson; Gen Larsson; Per Berglund; Andres Veide; Jan-Willem de Gier; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; AIDA-autotransporter; Escherichia coli; live vaccines; surface expression;

    Sammanfattning : The area of surface expression has gathered a lot of interest from research groups all over the world and much work is performed in the area. Autotransporters have been used for surface expression in Gram-negative bacteria. LÄS MER

  4. 3. Improved detection and performance of surface expression from the AIDA-I autotransporter

    Författare :Johan Jarmander; Gen Larsson; Ulrika Rova; Ines Ezcurra; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; surface expression; autotransport; AIDA-I; Escherichia coli; proteolysis; detection tag;

    Sammanfattning : Surface expression of recombinant proteins has attracted a lot of attention due to its potential in applications such as enzyme production, vaccine delivery and bioremediation. Autotransporters have been used for surface expression of a variety of proteins, but the expression systems reported in literature have typically been inflexible and incapable of detecting proteolysis, thereby limiting surface expression yield. LÄS MER

  5. 4. Tools for the development of intensified perfusion processes for mammalian cell culture

    Författare :Hubert Schwarz; Véronique Chotteau; Mark Smales; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Recombinant protein biomanufacturing; Perfusion process; Integrated continuous bioprocessing; Monoclonal antibody; Erythropoietin; Chinese Hamster Ovary cells; Human Embryonic Kidney cells; Perfusion medium optimization; Cell specific perfusion rate; Raman spectroscopy; N-glycosylation; Microbioreactors; Design of Experiments; Biotechnology; Bioteknologi;

    Sammanfattning : Recombinant protein therapeutics have become an indispensable part of modern medicine to treat a wide variety of diseases. Their manufacture is mostly based on mammalian cells with fed-batch production bioreactors and downstream batch purification unit operations. LÄS MER

  7. 5. Influence of electrode material and stochastic factors on the performance and microbial community assembly in microbial electrochemical systems

    Författare :Marie Abadikhah; Chalmers tekniska högskola; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; microbial community assembly; bioanode; biocathode; biochemical system;

    Sammanfattning : Microbial electrolysis cells (MECs) are systems with microbial communities in the form of biofilms on electrode surfaces. The electrogenic bacteria in the anode biofilm act as catalysts for the oxidization of organic compounds, leading to release of electrons, generation of electrical current, and production of hydrogen and methane at the cathode. LÄS MER