Sökning: "Biofysikalisk kemi"

Visar resultat 16 - 20 av 41 avhandlingar innehållade orden Biofysikalisk kemi.

1. 16. Conformational Studies of Biologically Active Peptides

   Författare :Björn Walse; Biofysikalisk kemi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; PapD chaperone; P pili; desmopressin; DDAVP; protein-peptide interactions; glycopeptides; peptide design; transferred NOE; molecular dynamics simulations; circular dichroism; peptide conformation; NMR spectroscopy; immunogenic glycopeptides; Physical chemistry; Fysikalisk kemi;

   Sammanfattning : In this work conformational studies have been performed on peptides from four different systems in an attempt to gain knowledge about their biological function. Circular dichroism (CD) spectroscopy and nuclear magnetic resonance (NMR) spectroscopy in combination with distance geometry and restrained molecular dynamics simulations were the methods used. LÄS MER

3. 17. Protein Dynamics Studied by NMR. Kinetics of the Adipocyte Fatty Acid-Binding Protein and Oligomerisation of the Low Molecular Weight Protein Tyrosine Phosphatase

   Författare :Tomas Åkerud; Biofysikalisk kemi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Physical chemistry; Fysikalisk kemi;

   Sammanfattning : Nuclear magnetic resonance spectroscopy (NMR) has been used to determine the kinetics of ligand binding to the adipocyte fatty acid-binding protein (A-FABP), as well as the oligomerisation and inter- and intramolecular dynamics of the low molecular weight-protein tyrosine phosphatase (LMW-PTP) in solution. NMR spectroscopy is sensitive to dynamic processes on timescales ranging from picoseconds to days. LÄS MER

4. 18. Membrane mediated aggregation of amyloid-β protein : a potential key event in Alzheimer's disease

   Författare :Marcus Bokvist; Gerhard Gröbner; Göran Lindblom; Lena Mäler; Umeå universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Alzheimer’s Disease; Aβ40; Circular Dichroism; NMR; Amyloids; Crowding; Peptide-Lipid Interaction; Biophysical chemistry; Biofysikalisk kemi;

   Sammanfattning : The pathogenesis of Alzheimer’s disease (AD), the most common senile dementia, is a complex process. A crucial event in AD is the aggregation of amyloid-β protein (Aβ), a cleavage product from the Amyloid Precursor Protein (APP). LÄS MER

5. 19. Protein structure dynamics and interplay : by single-particle electron microscopy

   Författare :Hans Elmlund; Hans Hebert; José Maria Carazo; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; biophysics; biochemistry; molecular; Biophysical chemistry; Biofysikalisk kemi;

   Sammanfattning : Single-particle cryo-electron microscopy (cryo-EM) is a method capable of obtaining information about the structural organization and dynamics of large macromolecular assemblies. In the late nineties, the method was suggested to have the potential of generating “atomic resolution” reconstructions of particles above a certain mass. LÄS MER

7. 20. On the quantitative analysis of electronic energy transfer/migration in proteins studied by fluorescence spectroscopy

   Författare :Mikael Isaksson; Lennart Johansson; Bo Albinsson; Umeå universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biophysical Chemistry; Biophysical chemistry; Biofysikalisk kemi;

   Sammanfattning : Two recently developed theories of electronic energy transfer/migration were for the first time applied to real protein systems for extracting molecular distances. The partial donor-donor energy migration (PDDEM) is an extension to the previously developed donor-donor energy migration (DDEM, F Bergström et al PNAS 96, 1999, 12477) which allows using chemically identical but photophysically different fluorophores in energy migration experiments. LÄS MER