Sökning: "Bengt Harald Jonsson"

Visar resultat 1 - 5 av 17 avhandlingar innehållade orden Bengt Harald Jonsson.

1. 1. Directed Evolution of Glutathione Transferases with Altered Substrate Selectivity Profiles : A Laboratory Evolution Study Shedding Light on the Multidimensional Nature of Epistasis

   Författare :Wei Zhang; Bengt Mannervik; Bengt-Harald Jonsson; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; glutathione transferase; azathioprine; directed evolution; semi-rational design; catalytic mechanism; saturation mutagenesis; reduced amino acid alphabets; molecular docking; protein evolution; multivariate data analysis; epistasis; fitness landscape; evolutionary trajectories; Biochemistry; Biokemi; Biokemi; Biochemistry;

   Sammanfattning : Directed evolution is generally regarded as a useful approach in protein engineering. By subjecting members of a mutant library to the power of Darwinian evolution, desired protein properties are obtained. Numerous reports have appeared in the literature showing the success of tailoring proteins for various applications by this method. LÄS MER

3. 2. Protein Engineering of Extracellular Superoxide Dismutase : Characterization of Binding to Heparin and Cellular Surfaces

   Författare :Ing-Marie Ahl; Lena Tibell; Bengt-Harald Jonsson; Steen Vang Petersen; Linköpings universitet; []
   Nyckelord :NATURAL SCIENCES; NATURVETENSKAP;

   Sammanfattning : Accumulating evidence indicates that oxygen free radicals are involved in many diseases and pathological conditions, such as aging, inflammation, reperfusion damage of ischemic tissue and various cardiovascular diseases. Extracellular superoxide dismutase (ECSOD) thus plays a major role in the maintenance of cells by providing protection against these toxic substances in the extracellular space. LÄS MER

4. 3. Improved Methods for Characterization of Protein Dynamics by NMR spectroscopy and Studies of the EphB2 Kinase Domain

   Författare :Alexandra Ahlner; Patrik Lundström; Bengt-Harald Jonsson; Lena Mäler; Linköpings universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES;

   Sammanfattning : Proteins are essential for all known forms of life and in many lethal diseases protein failure is the cause of the disease. To understand proteins and the processes they are involved in, it is valuable to know their structures as well as their dynamics and interactions. LÄS MER

5. 4. The Folding Energy Landscape of MerP

   Författare :Ann-Christin Brorsson; Bengt-Harald Jonsson; Daniel Otzen; Umeå universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; protein folding and stability; hydrogen exchange; intermediate; partial unfolding; Biokemi; Biochemistry; Biokemi; Biochemistry; biokemi;

   Sammanfattning : This thesis is based on studies, described in four papers, in which the folding energy landscape of MerP was investigated by various techniques. MerP is a water-soluble 72 amino acid protein with a secondary structure consisting of four anti-parallel β-strands and two α-helices on one side of the sheet in the order β1α1β2β3α2β4. LÄS MER

7. 5. SOD1´s Law : An Investigation of ALS Provoking Properties in SOD1

   Författare :Roberth Byström; Gerhard Gröbner; Stefan Marklund; Mikael Oliveberg; Bengt-Harald Jonsson; Umeå universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; ALS; amyotrophic lateral sclerosis; SOD1; protein folding; membrane interaction; aggregates; survival time; repulsive charge; Biochemistry; Biokemi;

   Sammanfattning : Proteins are the most important molecules in the cell since they take care of most of the biological functions which resemble life. To ensure that everything is working properly the cell has a rigorous control system to monitor the proper function of its proteins and sends old or dysfunctional proteins for degradation. LÄS MER