Sökning: "Aqueous two-phase system"

Visar resultat 21 - 25 av 30 avhandlingar innehållade orden Aqueous two-phase system.

1. 21. Spray-Dried Powders for Inhalation : Particle Formation and Formulation Concepts

   Författare :Jessica Elversson; Göran Alderborn; Anna Millqvist-Fureby; Lars Hovgaard; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Pharmaceutics; Spray drying; Particle formation; Density; Protein formulation; Encapsulation; Coating; Competitive adsorption; Polymer; ESCA; AFM; FTIR; Galenisk farmaci; Pharmaceutics; Galenisk farmaci;

   Sammanfattning : Spray drying is a method with a high potential in the preparation of protein particles suitable for pulmonary delivery. However, surface induced denaturation of bio-molecules during atomization and subsequent drying can be substantial and it is therefore important to develop new formulation concept for concurrent encapsulation and stabilization of proteins during spray drying. LÄS MER

3. 22. On the lateral organisation of the thylakoid membrane

   Författare :Ravi Danielsson; Biokemi och Strukturbiologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Thylakoid membrane heterogeneity; Plant biochemistry; Växtbiokemi; Thylakoid structure; Phase partition; Thylakoid fractions;

   Sammanfattning : In this work the lateral heterogeneity of the thylakoid membrane was investigated by quantifying the amount of photosystem I (PSI), photosystem II (PSII) and some PSII complexes and PSII proteins in different domains of the thylakoid. Grana, grana core, grana margins and stroma lamellae + end fractions were obtained by sonication and separation in a non-detergent, aqueous polymer two-phase system. LÄS MER

4. 23. Metal Binding Tags - Characterisation, Use in Bioseparation and Applications of Green Fluorescent Fusion Proteins

   Författare :Florent Bernaudat; Tillämpad biokemi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Proteiner; enzymologi; enzymology; E. coli; Immobilised metal ion affinity chromatography; Affinity tags; Green fluorescent protein; Surface plasmon resonance; Genetic engineering; Screening; Proteins; Immunoassay; Biotechnology; Bioteknik;

   Sammanfattning : Most of the recombinant proteins produced nowadays are fused to affinity tags, in order to facilitate their purification through affinity chromato-graphy. Out the different tags available, poly-histidine tags are among the most commonly used and can help purification through immobilised metal ion affinity chromatography (IMAC). LÄS MER

5. 24. LIPASES AND PHOSPHOLIPASES IN PHOSPHOLIPID SYNTHESIS

   Författare :Carmen Virto; Bioteknik; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; transphosphatidylation; acylation; vinyl ester; Phospholipid synthesis; lysophospholipid; kinetics; Biotechnology; Bioteknik;

   Sammanfattning : The synthesis of phospholipids with defined fatty acid and polar head composition catalysed by lipases and phospholipases was investigated. Lipases from Rhizopus arrhizus and Candida antarctica were used for the direct acylation of DL-a-glycerophosphate and L-a-glycerophosphatidylcholine, with different fatty acids. LÄS MER

7. 25. Amperometric Carbon Paste Biosensors Based on Horseradish Peroxidase and Oxidases

   Författare :Vijayakumar Rajendran; Centrum för analys och syntes; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Analytical chemistry; on-line monitoring; l-lactate; glucose; ethanol; Biosensor; Carbon paste; Analytisk kemi;

   Sammanfattning : Amperometric carbon paste peroxide sensors based on horseradish peroxidase (HRP) were developed. In the first approach, HRP was chemically modified with polyethylene glycol to improve the stability of the enzyme in the organic paste matrix and compared with native HRP electrode. LÄS MER