Sökning: "Antigen production"

Visar resultat 1 - 5 av 351 avhandlingar innehållade orden Antigen production.

1. 1. Production of polyclonal antibodies in rabbits and chickens immunised with human immunoglobulin G : A study of the utility of egg yolk antibody production as a substitute for rabbit serum antibody production

   Författare :Lise Svendsen Bollen; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Laboratory animals; antibody production; egg yolk; adjuvants; antibody response; avidity; Försöksdjursvetenskap; Laboratory animal science; Försöksdjursvetenskap; zoologisk utvecklingsbiologi; Animal Development;

   Sammanfattning : The utility of chicken egg yolk antibodies as an alternative to rabbit antisera was studied by comparing antibody and avidity development in immunised animals (15 rabbits and 30 chickens) during a one-year immunisation scheme. Human IgG was used as the model antigen and the efficacy of three adjuvants, Freund's Complete Adjuvant, Freund's Incomplete Adjuvant and Hunter's TiterMax, was compared. LÄS MER

3. 2. Regulation of experimentally induced airway obstruction

   Författare :Eva G. Lindström; Jan Lötvall; Linköpings universitet; []
   Nyckelord :airway obstruction; antigen; capsaicin; epithelium; inflammatory mediators; sensory neurons; tube preparation; tachykinins; formoterol; histamine; presynaptic inhibition; in vitro; guinea pig; MEDICINE; MEDICIN;

   Sammanfattning : Asthma is one of the commonest diseases in industrialized countries. The cause and mechanisms of !he disorder are still not fully unden;tood, al!hough it is known that inflammation in !he airways plays an important role. Inflammatory mediators, e.g. LÄS MER

4. 3. Characterization of Antigenic Properties and High Throughput Protein Purification

   Författare :Johanna Steen; Sophia Hober; Mats Ohlin; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; high throughput; protein purification; sample preparation; antigen solubility; immunogenicity; epitope mapping; antigen design; Bioengineering; Bioteknik; SRA - Molecular Bioscience; SRA - Molekylär biovetenskap;

   Sammanfattning : To understand the cellular processes, knowledge of the localization and function of proteins are essential. There are several high throughput ventures examining the human proteome. However, there are some bottlenecks in these ventures. For example the production and expression of soluble proteins for analysis. LÄS MER

5. 4. Avian IgY antibody : In vitro and in vivo

   Författare :David Carlander; Lennart Hammarström; Uppsala universitet; []
   Nyckelord :Medical sciences; Antigen production; chicken; cystic fibrosis; egg; ELISA; IgY; immunotherapy; Pseudomonas aeruginosa; yolk; MEDICIN OCH VÅRD; MEDICINE; MEDICIN; Clinical Chemistry; klinisk kemi;

   Sammanfattning : Immunoglobulin Y (IgY) is the major antibody found in eggs from chicken (Gallus domesticus). IgY can be used as an alternative to mammalian antibodies normally used in research, and its use in immunotherapy has recently been proposed. LÄS MER

7. 5. Mass Spectrometry and Affinity Based Methods for Analysis of Proteins and Proteomes

   Författare :Mårten Sundberg; Margareta Ramström; Jonas Bergquist; Christer Wingren; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Mass spectrometry; proteomics; microarray; protein; antibody; antigen; affinity; validation; Kemi med inriktning mot analytisk kemi; Chemistry with specialization in Analytical Chemistry;

   Sammanfattning : Proteomics is a fast growing field and there has been a tremendous increase of knowledge the last two decades. Mass spectrometry is the most used method for analysis of complex protein samples. It can be used both in large scale discovery studies as well as in targeted quantitative studies. LÄS MER