Sökning: "Antibody engineering"

Visar resultat 1 - 5 av 91 avhandlingar innehållade orden Antibody engineering.

  1. 1. Synthetic Paper for Point-of-Care Diagnostics : Capillary control, surface modifications, and their applications

    Författare :Weijin Guo; Wouter van der Wijngaart; Jonas Hansson; Govind V. Kaigala; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; capillary pump; lateral flow test; synthetic paper; OSTE; microarray; point-of-care; surface energy; viscosity; enrofloxacin; plasma separation; protein recovery rate; agglutination; antibody; glass; nitrocellulose; hydrophilic treatment; click chemistry; thiol-yne; thiol-maleimide; biotin-streptavidin; kapillärpump; lateral flödesprov; syntetiskt papper; OSTE; mikroarray; point-of-care; ytenergi; viskositet; enrofloxacin; plasmaseparation; proteinåtervinningshastighet; agglutination; antikropp; glas; nitrocellulosa; hydrofil behandling; klickkemi; tiol-yne; tiol-maleimid; biotin-streptavidin; Electrical Engineering; Elektro- och systemteknik;

    Sammanfattning : Capillary-based platforms for diagnostics are popular for the point-of-care diagnostics market because of their low cost, easy fabrication, and easy operation. Lateral flow tests are an excellent example of capillary-based platforms for point-of-care diagnostics. LÄS MER

  3. 2. Modeling Antibody Aggregation in Downstream Processing

    Författare :Frida Ojala; Avdelningen för kemiteknik; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Antibody; Aggregation; Downstream processing; Size exclusion chromatography; Reversible reaction model; General rate model;

    Sammanfattning : Antibodies are part of the immune system where their purpose is to recognize and neutralize foreign species such as viruses and bacteria, thus preventing and curing infections. The trend of using antibodies in the treatment of, for example, cancer is increasing. LÄS MER

  4. 3. Sequence- and structure guided engineering of proteins and enzymes for biotechnology and health applications

    Författare :Karen Schriever; Per-Olof Syrén; Elton P. Hudson; Eric A. Gaucher; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; protein engineering; enzymology; ancestral sequence reconstruction; antigen engineering; structural biology; terpene cyclases; enzyme thermodynamics; biocatalysis; phosphatases; allosteric regulation; Biotechnology; Bioteknologi;

    Sammanfattning : Proteins are highly diverse and sophisticated biomolecules that represent a cornerstone of biological structure and function and have been exploited in man-made applications for thousands of years. Those proteins that facilitate chemical reactions at physiologically relevant time-scales are referred to as enzymes. LÄS MER

  5. 4. Engineering Antibody Specificity Using Combinatorial Antibody Libraries

    Författare :Jenny Boström; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; antibody Antigen Interactions; Dual Specific Antibodies; Herceptin; Human Epidermal Growth Factor Receptor 2; Thermodynamic Analysis; therapeutic Antibody Engineering; Synthetic Phagedisplayed Antibody Libraries; Vascular Endothelial Growth Factor; Bacterial Display; trastuzumab;

    Sammanfattning : The research presented in this thesis investigates antibody specificity, antibody-antigen and protein-protein interactions using combinatorial antibody libraries, phage-display and bacterial display technology. One goal was to develop a method to generate antibodies that bind two protein antigens with high affinity using a single-antigen binding site, and to investigate the molecular and thermodynamic requirements for antibody multispecificity. LÄS MER

  7. 5. Strategies for Miniaturized Biomarker Detection

    Författare :Belinda Adler; Avdelningen för Biomedicinsk teknik; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; biomarker; miniturization; lab-on-a-chip; proteomics; PSA; microarray; mass spectrometry; MALDI MS; SPE; sample preparation; immunoaffinity; antibody; aptamer; mass tag; ISET.;

    Sammanfattning : The aim of this thesis is development and application of different miniaturized strategies for detection of biomarkers. The biomarker PSA (prostate specific antigen), which is prostate cancer specific, has been the main focus of the thesis. LÄS MER