Sökning: "Andrey Semenyuk"

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1. 1. Novel Methods for Synthesis of High Quality Oligonucleotides

   Författare :Andrey Semenyuk; Marek Kwiatkowski; Ulf Landegren; Joachim W. Engels; Uppsala universitet; []
   Nyckelord :Organic chemistry; oligonucleotide; solid-phase synthesis; RNA synthesis; phosphoramidite; abasic sites; depurination; 2 -O-DTM; protecting group; cartridge; conjugate; dithiomethyl linker; base-stable linker; oligonucleotide purification; nucleoside; Organisk kemi;

   Sammanfattning : The first part of the work describes a procedure of oligonucleotide purification using a reversed-phase cartridge. The developed method employs a very efficient yet mild oligonucleotide detritylation on the cartridge support allowing fast purification of oligonucleotides regardless of their 5´-modification. LÄS MER