Sökning: "Amplification facilitators"

Hittade 2 avhandlingar innehållade orden Amplification facilitators.

1. 1. Optimisation of diagnostic PCR a study of PCR inhibitors in blood and sample pretreatment

   Författare :Waleed Abu Al-Soud; Avdelningen för medicinsk mikrobiologi; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; mycology; virology; bacteriology; Microbiology; AmpliTaq Gold; Amplification facilitators; Betaine; Blood culture medium; Blood; BSA; cheese; faeces; gp32; Haemoglobin; Ions; Immunoglobulin G; Mikrobiologi; bakteriologi; virologi; mykologi;

   Sammanfattning : PCR is widely employed as a rapid, sensitive and specific molecular diagnostic technique in clinical diagnosis, environmental investigations and for monitoring biotechnical processes. However, the full potential of diagnostic PCR is limited, in part, by the presence of PCR-inhibitory substances in biological samples. LÄS MER

3. 2. Pre-PCR Processing Strategies for Quantitative Detection of Food-Borne Pathogens using Real-Time PCR

   Författare :Petra Wolffs; Teknisk mikrobiologi; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Livsmedelsteknik; Food and drink technology; DNA polymerase; sample treatment; pre-PCR processing; real-time PCR; Food-borne pathogens;

   Sammanfattning : Since the development of real-time PCR, the technology has been widely applied in the field of diagnostics. In comparison with conventional PCR it has opened up the possibility of accurate quantification of microorganisms in clinical, environmental and food samples. LÄS MER