Sökning: "Affinity two-phase partitioning"

Visar resultat 1 - 5 av 8 avhandlingar innehållade orden Affinity two-phase partitioning.

  1. 1. Isolation of caveolae using affinity two-phase partitioning

    Författare :Parisa Abedinpour; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; metabolism; Biokemi; Metabolism; Biochemistry; basolateral; apical; immunoaffinity; wheat germ agglutinin; caveolin; caveolae caveolae enriched fraction; plasma membranes; rat lung; rat liver; Affinity Partitioning; two-phase partitioning;

    Sammanfattning : The intention of this work was to establish alternative purification methods to obtain highly purified caveolae from various tissues. In order to isolate caveolae, sufficiently pure plasma membranes are needed. LÄS MER

  3. 2. Affinity Two-Phase Partitioning of Liposomes and Membranes

    Författare :Irene Barinaga-Rementeria Ramírez; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; miniaturised two-phase system; levitated drop; biotin; avidin; antibody; immunoaffinity; purification; rat lung; rat liver; caveolae; membrane; Affinity two-phase partitioning; liposome; Metabolism; Biochemistry; Biokemi; metabolism;

    Sammanfattning : Affinity two-phase partitioning based on an immunoaffinity sandwich approach for the rapid and selective purification of membranes is presented in this thesis. The method was developed by studying different parameters governing the affinity partitioning of model membranes. To this end, biotinylated liposomes were used. LÄS MER

  4. 3. Selective extraction systems for detection and purification of membrane proteins

    Författare :Ulf Sivars; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; partitioning; Aqueous two-phase systems; separation; membrane proteins; detergent; micelle; polymer; Biochemistry; affinity; Metabolism; Biokemi;

    Sammanfattning : Increased understanding of membrane proteins is important for characterization of biochemical processes. Improved membrane protein isolation methods is a key issue for effective functional and structural determination of the large amount of unknown membrane proteins. LÄS MER

  5. 4. Separation Methods in Membrane Proteomics -Integration of Aqueous Two-Phase Partitioning for Enrichment and Pre-Fractionation

    Författare :Henrik Everberg; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Membrane proteins; Proteins; metabolism; Biokemi; Metabolism; Biochemistry; Aqueous two-phase partitioning; enzymology; Proteiner; enzymologi; Proteomics;

    Sammanfattning : The study of the protein complement of a genome is called proteomics. Proteomics can be defined as identification, quantification and characterization of a total set of proteins expressed at different time-points or under different cellular states to provide global information about biological processes. LÄS MER

  7. 5. Miniaturised Bioanalytical Chemistry in Acoustically Levitated Droplets

    Författare :Sabina Santesson; Centrum för analys och syntes; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Analytical chemistry; Analytisk kemi; protein crystallisation; affinity two-phase partitioning; single cell analysis; bioanalytical chemistry; airborne chemistry; levitated droplets; Miniaturisation; acoustic levitation;

    Sammanfattning : Fuelled by the current demands of the biological sciences, there is today a growing interest in microscale analysis. Miniaturisation of analytical instruments has become a long-standing trend. Miniaturisation offers several advantages, including rapid separation times, high performance and throughput, reduced costs, etc. LÄS MER