Sökning: "Affinity chromatography"

Visar resultat 21 - 25 av 128 avhandlingar innehållade orden Affinity chromatography.

  1. 21. Mammalian brain acetylcholinesterase : a study of its solubilization, purification, molecular state and interactions with cholinergic ligands including an endogenous modulator

    Författare :Bertil Niklasson; Umeå universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Affinity chromatography; caudate nucleus; acetylcholinesterase; tetracaine; décaméthonium; d-tubocurarine; edrophonium; fluoride ion; hysteric properties; endogenous ligands to AChE;

    Sammanfattning : Membrane bound AChE from calf brain caudate nucleus was solubilized by use of ion-free media in presence of 10~ M EDTA and 10“5M tetracaine. The irreversible release of AChE was more effectively inhibited by divalent ions compared to monovalent ions added to the medium. LÄS MER

  3. 22. Genetically Engineered Plants: Improved stress tolerance, growth and facilitated protein purification

    Författare :Gösta Lilius; Tillämpad biokemi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; glycine betaine; gene; freezing; flooding; drought; dicoteledones; desiccation; compatible solutes; cold; choline dehydrogenase; chill; CDH; betaine; betA; alkaloids anoxia; affinity purification; affinity tags; handles; hemoglobin; hypoxia; low temperatu; Biotechnology; Bioteknik;

    Sammanfattning : Heterologous genes were expressed in plants in which the overall goal was to increase the value of the final crop. Various aspects were explored and evaluated, such as improving the stress tolerance, enhancing the availability of oxygen in growing cells and producing rare proteins with increased degrees of purity. LÄS MER

  4. 23. Purification Processes for Complex Biomacromolecules

    Författare :Hans Blom; Helena Grennberg; Mats Lundgren; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Chromatography; enzymes; mucins; fractionation; plasmid DNA; analysis; clarification; process design; Chemistry with specialization in Surface Biotechnology; Kemi med inriktning mot ytbioteknik;

    Sammanfattning : This thesis details various techniques and considerations for the purification of complex biomacromolecules. Initially an α-mannosidase from babaco fruit was purified using anion exchange-, lectin affinity- and size exclusion chromatography. LÄS MER

  5. 24. Affibody ligands in immunotechnology applications

    Författare :Jenny Rönnmark; KTH; []
    Nyckelord :phage display; combinatorial; affinity; IgA ligand; immunohistochemistry; affibody-fusions;

    Sammanfattning : This thesis describes the development and use ofnon-immunoglobulin affinity proteins denoted affibodies asalternatives to antibodies in different immunotechnologyapplications. A 58 aa IgG Fc binding three-helix bundle domainZ, derived from staphylococcal protein A has been used asframework for library constructions, in which the face of themolecule involved in the native binding activity has beenengineered by combinatorial protein engineering. LÄS MER

  7. 25. Versatile Implementations of an Improved Cell-Free System for Protein Biosynthesis : Functional and structural studies of ribosomal protein L11 and class II release factor RF3. Novel biotechnological approach for continuous protein biosynthesis

    Författare :Lamine Bouakaz; Måns Ehrenberg; Richard Buckingham; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Molecular biology; Protein synthesis; translation termination; ribosome; release factor; cell-free system; missense error; gel filtration; affinity chromatography; Molekylärbiologi; Molecular biology; Molekylärbiologi;

    Sammanfattning : Advances in genetics, proteomics and chromatography techniques have enabled the successfully generation of a cell-free bacterial translation system composed of highly pure and active components. This system provided an ideal platform for better elucidating the mechanism of each individual step of the prokaryotic protein biosynthesis and the function of the translation factors involved in the process. LÄS MER