Sökning: "3HB"

Visar resultat 1 - 5 av 7 avhandlingar innehållade ordet 3HB.

  1. 1. Strategies for improved Escherichia coli bioprocessing performance

    Författare :Johan Jarmander; Gen Larsson; Thomas Egli; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; E. coli; Salmonella; surface expression; autotransport; AIDA-I; lignocellulose; glucose; xylose; arabinose; simultaneous uptake; 3HB; Bioteknologi; Biotechnology;

    Sammanfattning : Escherichia coli has a proven track record for successful production of anything from small molecules like organic acids to large therapeutic proteins, and has thus important applications in both R&D and commercial production. The versatility of this organism in combination with the accumulated knowledge of its genome, metabolism and physiology, has allowed for development of specialty strains capable of performing very specific tasks, opening up opportunities within new areas. LÄS MER

  3. 2. Engineering short-chain carboxylic-acid metabolism in the model microorganism Escherichia coli

    Författare :Gustav Sjöberg; Antonius J. A. van Maris; Solange Mussatto; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; metabolic engineering; R -3-hydroxybutyrate; volatile fatty acids; bioprocess design; Escherichia coli; bacterial artificial chromosome; Bioteknologi; Biotechnology;

    Sammanfattning : The ever-increasing concern about carbon dioxide emissions has created an urgent need to develop alternative methods to cheaply and renewably produce materials, chemicals and fuels. The biorefinery is uniquely suited to deliver these products from sustainable biomass. LÄS MER

  4. 3. Strain- and bioprocess-design strategies to increase production of (R)-3-hydroxybutyrate by Escherichia coli

    Författare :Mónica Guevara-Martínez; Gen Larsson; Antonius J. A. van Maris; Pau Ferrer; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; E. coli; R -3-hydroxybutyrate; metabolic engineering; bioprocess design; NADPH; acetic acid; thioesterase; BAC; Bioteknologi; Biotechnology;

    Sammanfattning : Microbial bio-based processes have emerged as an alternative to replace fossil-based processes for the production of fuels and chemicals. (R)-3-hydroxybutyrate (3HB) is a medium-value chemical that has gained special attention as a precursor of antibiotics and vitamins, as a monomer for the synthesis of tailor-made polyesters and as a nutritional source for eukaryotic cells. LÄS MER

  5. 4. Metabolic engineering and cultivation strategies for recombinant production of (R)-3-hydroxybutyrate

    Författare :Mariel Perez-Zabaleta; Gen Larsson; Antonius J. A. van Maris; Diethard Mattanovich; KTH; []
    Nyckelord :Escherichia coli; R -3-hydroxybutyrate; nitrogen limitation; nitrogen depletion; lignocellulose; fed batch; acetate; β-ketothiolase; acetoacetyl-CoA reductase; Halomonas boliviensis.; Bioteknologi; Biotechnology;

    Sammanfattning : Metabolic engineering and process engineering are two powerful disciplines to design and improve microbial processes for sustainable production of an extensive number of compounds ranging from chemicals to pharmaceuticals. The aim of this thesis was to synergistically combine these two disciplines to improve the production of a model chemical called (R)-3-hydroxybutyrate (3HB), which is a medium-value product with a stereocenter and two functional groups. LÄS MER

  7. 5. Influence of processing and processing additives on the degradation of aliphatic polyesters

    Författare :Rasmus Renstad; KTH; []
    Nyckelord :;

    Sammanfattning : Poly(3-hydroxybutyrate-co-3-hydroxyvalerate), P(3HB-co-3HV),poly(ε-caprolactone), PCL, and poly(lactic acid), PLA,have been subjected to film-blowing, melt-extrusion andmelt-pressing. The influence of processing conditions andprocessing additives (i.e. plasticizer, antiblocking and slipagents) on properties and degradation have been studied. LÄS MER