Sökning: "3-hydroxybutyrate"

Visar resultat 1 - 5 av 19 avhandlingar innehållade ordet 3-hydroxybutyrate.

1. 1. Production of poly(3-hydroxybutyrate) and ectoines using a halophilic bacterium

   Författare :Thuoc Doan Van; Bioteknik; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Fermentation; Ectoine; halophilic bacterium; Poly 3-hydroxybutyrate ;

   Sammanfattning : Halomonas boliviensis is a moderate halophilic bacterium, isolated from a soil sample around a hypersaline lake Laguna Colorada in the Andean region of Bolivia. The organism is capable of growing over a wide range of NaCl concentration (0-25 %, w/v), however with an optimum of about 4.5 % (w/v) NaCl. LÄS MER

3. 2. Metabolic engineering and cultivation strategies for recombinant production of (R)-3-hydroxybutyrate

   Författare :Mariel Perez-Zabaleta; Gen Larsson; Antonius J. A. van Maris; Diethard Mattanovich; KTH; []
   Nyckelord :Escherichia coli; R -3-hydroxybutyrate; nitrogen limitation; nitrogen depletion; lignocellulose; fed batch; acetate; β-ketothiolase; acetoacetyl-CoA reductase; Halomonas boliviensis.; Bioteknologi; Biotechnology;

   Sammanfattning : Metabolic engineering and process engineering are two powerful disciplines to design and improve microbial processes for sustainable production of an extensive number of compounds ranging from chemicals to pharmaceuticals. The aim of this thesis was to synergistically combine these two disciplines to improve the production of a model chemical called (R)-3-hydroxybutyrate (3HB), which is a medium-value product with a stereocenter and two functional groups. LÄS MER

4. 3. Production of Ectoines and Poly(3-Hydroxybutyrate): High Cell-Density Cultivation of Halomonas Boliviensis

   Författare :Hector Guzmán; Bioteknik; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; poly 3-hydroxybutyrate ; hydroxyectoine; ectoine; Halomonas boliviensis; compatible solutes; Fed-batch cultivation;

   Sammanfattning : This thesis reports a study involving the use of Halomonas boliviensis, a moderate halophile, for production of biopolyester poly(3-hydroxybutyrate) (PHB) and compatible solutes ectoine and hydroxyectoine through high cell-density cultivation comprising fed-batch cultures. PHB is a biodegradable plastic produced by bacteria as energy reserve and can potentially be used as a replacement of fossil based plastic. LÄS MER

5. 4. Strain- and bioprocess-design strategies to increase production of (R)-3-hydroxybutyrate by Escherichia coli

   Författare :Mónica Guevara-Martínez; Gen Larsson; Antonius J. A. van Maris; Pau Ferrer; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; E. coli; R -3-hydroxybutyrate; metabolic engineering; bioprocess design; NADPH; acetic acid; thioesterase; BAC; Bioteknologi; Biotechnology;

   Sammanfattning : Microbial bio-based processes have emerged as an alternative to replace fossil-based processes for the production of fuels and chemicals. (R)-3-hydroxybutyrate (3HB) is a medium-value chemical that has gained special attention as a precursor of antibiotics and vitamins, as a monomer for the synthesis of tailor-made polyesters and as a nutritional source for eukaryotic cells. LÄS MER

7. 5. Halomonas species as a source of poly(3-hydroxybutyrate). Preparation of hydrophilic macroporous polymer scaffolds

   Författare :Daniel Guzmán; Bioteknik; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Halomonas species; poly 3-hydroxybutyrate ; genome sequence; Fed-batch cultivation; macroporous scaffolds;

   Sammanfattning : This thesis presents studies on moderately halophilic bacteria belonging to the family Halomonadaceae that accumulate polyhydroxybutyrate (PHB) in high amounts when grown under conditions of nitrogen limitation. PHB is the simplest homopolymer belonging to the class of polyhydroxyalkanoates (PHAs), the biopolyesters that can biodegraded in the environment to carbon dioxide and water and are hence attractive substitutes for fossil-derived plastics. LÄS MER