Initiation of Innate Immune Responses in the Freshwater Crayfish Pacifastacus leniusculus

Sammanfattning: Prophenoloxidase (proPO) is a key enzyme for generation of melanin and is activated by the proPO activating enzyme (ppA) to its active form, PO. The active ppA was purified and cloned from crayfish hemocytes and it is a typical serine proteinase containing a clip, a proline-rich, and a glycine-rich domain. A recombinant protein containing the clip-domain, with homology to horseshoe crab big defensin and mammalian