Plant biology through quantitative proteomics

Sammanfattning: Over the last decade the field of mass spectrometry based proteomics has advanced from qualitative, analyses leading to publications revolving around lists of identified proteins and peptides, to addressing more biologically relevant issues requiring measurement of the abundance of identified proteins and hence quantitive mass spectrometry. The work described in this thesis addresses problems with quantitive proteomics in plant sciences, particularly complications caused by the complexity of plant proteomes (generated by genomic duplications), which makes mass spectrometry-based based proteomic analyses more difficult than in mammalian species. In order to understand complex biological processes it is vital to analyse the participating molecules with as little bias as possible. Strategies for minimizing and maximizing the acquired information in proteomic investigations of plants are presented in the appended papers and discussed in the thesis.

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