Stimuli-responsive lipid bilayer mimics for protein, virus and cell recognition

Sammanfattning: The most well-studied two-dimensional biomimetic cellular membrane modelsare self-assembled monolayers (SAMs) and supported lipid bilayers (SLBs).The former has the advantage of control over ligand density, homogeneity andorientation, allowing unambiguous interaction studies. It however lacks longrangelateral mobility, which is one of the most important aspects of cellularmembranes. SLBs are laterally mobile but they are fragile and instable uponexposure to air. Literature examples that contain all the above desirablecharacteristics with stimuli-responsiveness to fabricate biomaterials forbiosensing or modulating cell adhesion are rare. We here report on anadaptable platform, reversible self-assembled monolayers (rSAMs), featuringstrongly enhanced affinity towards influenza viruses as compared to SAMs,lateral mobility to investigate glycan-lectin interactions and tunable surfacedynamics to modulate cell adhesion. This new system utilizes noncovalentamidinium-carboxylate ion pairs for building up stable and ordered twodimensionalassemblies, akin to lipid bilayers but with a simple preparationprocess, stimuli-responsiveness and fast on/off rates.