Tools in biocatalysis enzyme immobilisation on silica and synthesis of enantiopure amines

Sammanfattning: This thesis presents two techniques in the field of biocatalysis:An enzyme immobilisation method based on the His6-tag for attachment on modified silica oxide beads, and it’s employment in aqueous and organic medium for synthesis applications. The method functions as a one step extraction and immobilisation protocol.An equilibrium displacement system which enables complete conversion in reactions with ?-transaminases where isopropylamine is the donor, a route for synthesis of pharmaceutically interesting enantiopure amines.Biocatalysis is predicted to be a paramount technology for an environmentally sustainable chemical industry, to which every newly developed method represents a small but important step. The work done here is aimed to be a part of this development.