Perspectives on urban wastewater as a source of microbial pollution
Sammanfattning: Wastewater treatment plants are important links for dissemination of intestinal bacteria into surface waters. In this study, twelve mallards were exposed to treated wastewater for a period of 55 days. Faecal samples were collected and analysed for Enterococcus spp. and C. jejuni. In general, the mallard and wastewater enterococci isolates belonged to different phenotypes, although some strains were identical. Phenotypical characteristics of C. jejuni, including antibiotic resistance, and genetical (PFGE and MLST) patterns were compared. All STs have previously been found in both humans and wild birds. The phenotypical expression of resistance against ampicillin and cefazolin, and ability to assimilate malate and succinate, changed during the mallards exposure to wastewater. Edible clams were collected in Maputo Bay during both the dry and rainy seasons, and number of viable counts of V. parahaemolyticus peaked during the rainy season. A high percentage showed haemolytic capacity but did not carry the standard set of virulence genes. The persistence of E. faecium and E. faecalis strains in sterilized treated wastewater at 10°C and 20°C was evaluated, including if ciprofloxacin had any effect. We could conclude that E. faecalis had a lower DC10 (92 and 43 days) than E. faecium (333 and 68 days) at 10°C and 20°C, respectively. Most of the strains were unaffected of ciprofloxacin was, but there were exceptions. All strains remained culturable the whole studied period (108 days).
Denna avhandling är EVENTUELLT nedladdningsbar som PDF. Kolla denna länk för att se om den går att ladda ner.