Phylogeny and Taxonomy of Childia (Acoela) New characters for unraveling acoel phylogenies from molecules, ultrastructure, immunocytochemistry and confocal microscopy

Sammanfattning: This thesis presents a comprehensive phylogenetic and taxonomic study of an acoel subgroup, the Childiidae. Members of this taxon are characterized by well-developed male copulatory organs with conical/cylindrical stylets. The phylogenetic analyses, by means of total evidence approach, based on three molecular markers (18S and 28S rRNA genes and Histone H3) and 50 morphological characters reaffirm the non-monophyly of the Childiidae sensu Dörjes, 1968 (Actinoposthiidae and Childia+Paraphanostoma). The total evidence phylogeny strongly support the Childia+Paraphanostoma clade separate from other former members of the Childiidae, which are now placed in Actinoposthiidae. The monophyly of Childia+Paraphanostoma is well corroborated by several morphological characters. A new taxon Childia is defined, in accordance with the PhyloCode, comprising all former Paraphanostoma species and a member of the monotypic genus C. groenlandica. A new diagnosis for the current members of Childia is provided.Several structures, shown to hold promising phylogenetic signals for unraveling acoel relationships, such as musculature pattern, sperm and male copulatory organs, are investigated, using a combination of traditional and modern techniques (ultrastructure, immunocytochemistry and confocal microscopy), with main focus on Childia and its closest relatives. New characters are described and their phylogenetic significance assessed. Morphological characters relating to body-wall musculature, statocyst muscles, male copulatory organ musculature and ultrastructure, and sperm cytoplasmic granules are shown to carry important phylogenetic signals at lower taxonomic levels, while most of the characters related to sperm ultrastructure are useful at higher taxonomic levels within the Acoela. The data obtained undermine the phylogenetic use of the seminal bursa in Childia. In addition to this, it is shown that most of the classical morphological characters used in acoel taxonomy, obtained using traditional histological methods, may be misleading in identifying monophyletic entities within the Acoela.The most corroborated synapomorphies, identified in this thesis, are used in determining the taxonomic placement of a new viviparous acoel, Childia vivipara, into the taxon Childia.