Who is killing whom? Hantaviruses vs programmed cell death

Sammanfattning: Hantaviruses belong to the Bunyaviridae family of negative stranded RNA viruses. They carry a tri-segmented genome and consist of four structural proteins. The four structural proteins are two glycoproteins Gn and Gc, a nucleocapsid (N) protein and an RNA-dependent RNA-polymerase. An additional nonstructural protein can be expressed by some hantaviruses. Hantavirus-infection cause two severe diseases in humans with potential deadly outcome, namely hemorrhagic fever with renal syndrome (HFRS) or hantavirus cardiopulmonary syndrome (HCPS). One main target for hantavirus-infection is the endothelial cells and vascular leakage is a hallmark for both HFRS and HCPS. In patients strong cytotoxic lymphocyte responses are seen. Cytotoxic lymphocytes, such as natural killer (NK) cells and cytotoxic T lymphocytes, cause apoptosis in virus-infected cells via the cytotoxic granule pathway or the death receptor pathway. The cytotoxic granule pathway uses granzyme B to facilitate programmed cell death (PCD) in the target. The death receptor pathway uses death ligands, among them tumor necrosis factor related apoptosis-inducing ligand (TRAIL) binds to death receptor (DR) 4 or 5, to induce PCD. One potential mechanism regarding hantavirus pathogenesis might be killing of infected endothelial cells by cytotoxic lymphocytes, thus causing leakage of the endothelium. This is contradicted by findings showing that in patient autopsy, hantavirus-infected cells are intact. The aim of this PhD thesis is to give a possible explanation of this dichotomy and to better understand hantavirus pathogenesis. The first part of this thesis (paper I and II) shows that hantavirus-infection protects cells from cytotoxic lymphocytes via inhibiting granzyme B activity and by down-regulating DR5 from the cell surface. Granzyme B and caspase 3, enzymes needed for apoptosis (a type of PCD), both interacts with hantavirus N protein, and they are both inhibited by the N protein. Further, hantavirus-infection of primary endothelial cells causes miss-localization of DR5. In infected cells DR5 is found in the nucleus instead of on the cell surface. Taken together, hantavirus-infection blocks the two major pathways used by cytotoxic lymphocytes to induce cell death, suggesting that hantavirus pathogenesis is not due to killing of infected cells by cytotoxic lymphocytes. The last part of this thesis (paper III) focuses on hantavirus activated NK cell mediated killing of uninfected endothelial cells. NK cells co-incubated with hantavirus-infected endothelial cells are activated. This activation is contact dependent and was attributed to IL-15 and IL-15Rα expression on hantavirus-infected cells’ surface. Interestingly, these activated NK cells induce cell death in uninfected cells with normal HLA class I levels, indicating that hantavirus might cause NK cell mediated killing of uninfected bystander cells. Taken together, the papers I, II and III included in this thesis shows that hantavirusinfection protects cells from cytotoxic lymphocyte mediated killing, while infected cells can cause NK cell activation and possibly subsequent NK cell killing of uninfected cells.http://kib.ki.se/en/publish-analyse/open-access