Sökning: "thermostable"

Visar resultat 1 - 5 av 38 avhandlingar innehållade ordet thermostable.

  1. 1. Glycoside hydrolases from Rhodothermus marinus Modular organisation and structure-function relationships

    Författare :Maher Abou-Hachem; Bioteknik; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; calcium-binding; modular; endoglucanase; thermostable; CBM; xylanase; Biotechnology; Bioteknik;

    Sammanfattning : The thermophilic bacterium Rhodothermus marinus produces several thermostable glycoside hydrolases. The studies presented in this thesis were performed on two enzymes, belonging to glycoside hydrolase families 10 and 12, produced by this microorganism. The family 10 xylanase, Xyn10A, is modular in architecture consisting of five domains or modules. LÄS MER

  3. 2. Glycoside hydrolases from extremophiles isolated in Iceland. alpha-L-Rhamnosidases and pectin degrading enzymes

    Författare :Hakon Birgisson; Bioteknik; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; immobilization; Biotechnology; Bioteknik; crystallization; sequencing; cloning; screening; thermophile; psychrotroph; psychrophile; alpha-L-arabinofuranosidase; alpha-L-rhamnosidase; thermostable; cold active; polygalacturonase;

    Sammanfattning : Extreme environments (high and low temperature, high and low pH, etc.) are inhabited by a vast and diverse flora of microorganisms, which are adapted to these environments (extremophiles). LÄS MER

  4. 3. Exploring thermostable glycoside hydrolases: Amylases and beta-glucosidases

    Författare :Pernilla Turner; Bioteknik; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Bioteknik; Biotechnology; Proteiner; beta-glucosidase; thermostability; activity; cyclodextrinase; enzymology; crystallization; enzymologi; expression; glycoside hydrolase; Proteins; transglycosylation;

    Sammanfattning : Two putative cyclodextrinases (CDases) were amplified from two moderate thermophiles isolated in Iceland: Anoxybacillus flavithermus and Laceyella sacchari. They were cloned, expressed and characterized. The expression of the former was optimized in E. LÄS MER

  5. 4. Diversity of thermostable DNA enzymes from Icelandic hot springs

    Författare :Sigridur Hjorleifsdottir; Bioteknik; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; thermophiles; microbial diversity; SSU rRNA; Thermus; hot springs; Biotechnology; Bioteknik; Biochemistry; enzymologi; Proteiner; enzymology; metabolism; Proteins; DNA enzymes; Biokemi;

    Sammanfattning : The diversities of bacteria as well as thermostable DNA enzymes in Icelandic hot springs were studied using different techniques. First of all the bacterial diversity of different microbial mats as well as sediment samples was described by using both molecular methods and cultivation methods. LÄS MER

  7. 5. Thermostable glycosidases and glycosynthases as biocatalysts in green chemistry applications

    Författare :Tania Pozzo; Bioteknik; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Green chemistry; 3D sturcture; Glycosyl hydrolases; Glycosynthases; Alkyl glucosides; Oligosaccharides; Flavonoids;

    Sammanfattning : The constant need to develop environmental friendly processes using renewable raw materials and to reduce harmful waste production motivates the present investigation focusing on biocatalyst development for enzymatic synthesis, a biotechnological approach in the field of green chemistry. At first, structural functional experiments were made for a β-glucosidase from T. LÄS MER