Sökning: "solution conformational analysis"

Visar resultat 11 - 15 av 31 avhandlingar innehållade orden solution conformational analysis.

  1. 11. Structural characterization of Deinococcus radiodurans phytochrome in solution

    Författare :Lidija Vrhovac; Sebastian Westenhoff; Vladislav Orekhov; Magnus Wolf-Watz; Uppsala universitet; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; Biokemi; Biochemistry;

    Sammanfattning : Phytochromes are red/far-red photoreceptors which control a wide spectrum of biological processes inplants, fungi, and bacteria. Although phytochromes have been extensively studied, their mechanism ofaction still remains elusive. The signaling mechanism suggested by crystal structures involves refoldingof the so-called PHY tongue. LÄS MER

  3. 12. Synthesis and analysis of carbohydrates related to bacterial polysaccharides

    Författare :Hani Mobarak; Göran Widmalm; Ulf Nilsson; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; carbohydrates; synthesis; conformation; NMR; O-antigen; computer-assisted structure elucidation; Organic Chemistry; organisk kemi;

    Sammanfattning : The outer surface of bacteria is composed of around 75% carbohydrates, which are vital for the bacteria to survive and communicate with the host biological system. The thesis discusses different properties of carbohydrates that are essential for understanding the bacterial behavior in biological systems. LÄS MER

  4. 13. Structural and conformational studies of bacterial polysaccharides employing NMR spectroscopy

    Författare :Mikael Staaf; Jesus Jiminez-Barbero; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Bacterial polysaccharides; Magnetic resonance spectroscopy; Organic chemistry; Organisk kemi; Organic Chemistry; organisk kemi;

    Sammanfattning : Methods and approaches for performing structural and conformational analysis of bacterial polysaccharides in solution are described. The structure of one exopolysaccharide from Lactobacillus helveticus and two O-antigens from Escherichia coli have been determined using NMR spectroscopy, chemical degradation and mass spectrometry. LÄS MER

  5. 14. Surface Properties of Antibodies and their Complexes with Antigens Studied by LLPC

    Författare :Christer Wingren; Institutionen för immunteknologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; ligand-binding.; surface properties; isomerism; conformational changes; antibody; specificity; two-phase partitioning; antigen-binding sites; Biochemistry; Metabolism; Biokemi; metabolism;

    Sammanfattning : The main objective of this thesis was to gain further insight into the relationship between the exposed surfaces and the functional properties of antibodies and their complexes with antigens in solution. To study this relationship, a new technique, liquid-liquid partition chromatography (LLPC) in aqueous two-phase systems, was developed further with respect to sensitivity, selectivity and reproducibility. LÄS MER

  7. 15. Exploring the structure of oligo- and polysaccharides : Synthesis and NMR spectroscopy studies

    Författare :Hanna Jonsson; Göran Widmalm; Antonio Molinaro; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Carbohydrates; NMR spectroscopy; synthesis; lipopolysaccharides; conformational studies; Organic chemistry; Organisk kemi; organisk kemi; Organic Chemistry;

    Sammanfattning : A deeper understanding of the diversity of carbohydrates and the many applications of oligo- and polysaccharides found in nature are of high interest. Many of the processes involving carbohydrates affect our everyday life. This thesis is based on six papers all contributing to an extended perspective of carbohydrate property and functionality. LÄS MER