Sökning: "single-cell viability"

Visar resultat 1 - 5 av 15 avhandlingar innehållade orden single-cell viability.

  1. 1. Live Single Cell Imaging and Analysis Using Microfluidic Devices

    Författare :Mohammad Ali Khorshidi; Helene Andersson-Svahn; Håkan Jönsson; Carolina Wählby; Johan Elf; KTH; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Single cell analysis; time-lapse fluorescence imaging; automated image analysis; microwell; droplet microfluidics; NK cells; single cell tracking; migration behavior analysis; cell-cell interaction; optical microscopy; image analysis; image processing; microfluidics; immune cells; tracking; counting; morphology analysis;

    Sammanfattning : Today many cell biological techniques study large cell populations where an average estimate of individual cells’ behavior is observed. On the other hand, single cell analysis is required for studying functional heterogeneities between cells within populations. LÄS MER

  3. 2. Droplet microfluidics for single cell and nucleic acid analysis

    Författare :Prem Kumar Periyannan Rajeswari; Helene Andersson Svahn; Håkan Jönsson; Martin Dufva; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Acoustophoresis; Biomarker detection; Cell behavior analysis; Cell factories; Droplet microfluidics; Droplet PCR; High throughput biology; Label-free enrichment; Single cell analysis; Bioteknologi; Biotechnology;

    Sammanfattning : Droplet microfluidics is an emerging technology for analysis of single cells and biomolecules at high throughput. The controlled encapsulation of particles along with the surrounding microenvironment in discrete droplets, which acts as miniaturized reaction vessels, allows millions of particles to be screened in parallel. LÄS MER

  4. 3. Micro-scale Platforms for Investigations of Biological Structures

    Författare :Anna Kim; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; microfabrication; non-ionic surfactants; single-cell viability; hydrodynamic flow confinement; cell-to-cell connections; SU-8; Teflon AF; cell protrusions; free-standing probes; millimeter-waves;

    Sammanfattning : This thesis describes the development of innovative micro-scale platforms as a means to address the current challenges within the field of cell biology, and to provide an interface between miniaturized analytical technology and experimental systems for the life sciences. The work includes an analytical platform for rapid assessment of the viability of adherent single cells; a surface patterning strategy for investigations of cell-to-cell communication via protrusions; a versatile microfluidic probe in SU-8 hard-polymer, and a microfabricated millimeter-wave measurement system for the identification and characterization of niosome (non-ionic surfactant vesicle) constituents, which is operated in a label-free, non-invasive manner. LÄS MER

  5. 4. New insights on type 2 immunity key drivers : mast cells and group 2 innate lymphoid cells

    Författare :Avinash Ravindran; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : Type 2 immune responses are characterized by the production of cytokines IL-4, IL-5 and IL-13. They are known to have crucial physiological and pathophysiological functions in the body. Data from recent studies indicate their importance in the containment and clearance of helminth infections. LÄS MER

  7. 5. Mathematical Modelling of Cellular Ageing: a Multi-Scale Perspective

    Författare :Barbara Schnitzer; Göteborgs universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; replicative ageing; healthy ageing; yeast; complex systems; multi-scale model; hybrid model; flux balance analysis; ordinary differential equations; Boolean modelling;

    Sammanfattning : In a growing and increasingly older population, we are progressively challenged by the impact of ageing on individuals and society. The UN declared the years 2021-2030 as the Decade of Healthy Ageing, highlighting the efforts to minimise the burden of ageing and age-related diseases. LÄS MER