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Visar resultat 1 - 5 av 46 avhandlingar som matchar ovanstående sökkriterier.

  1. 1. Biosynthesis of capsaicinoids by recombinant Saccharomyces cerevisiae

    Författare :Nina Muratovska; Teknisk mikrobiologi; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; capsaicinoids; nonivamide; baker’s yeast; Capsicum; Saccharomyces cerevisiae; Pseudomonas putida; lignin-derived substrates; vanillin aminotransaminase; reductive amination; vanillylamine; N-Acyltransferase; CoA-ligase; amide; whole-cell bioconversion; TRPV1 receptor; ion channel; calcium dye Fluo-4; flow cytometry;

    Sammanfattning : Throughout history, people have used products from different microorganisms and plants for many medicinal and nutritional applications. Chilli peppers have been used for spicing up food, but also for their pharmacological properties. LÄS MER

  3. 2. Improving initial xylose metabolism in recombinant Saccharomyces cerevisiae

    Författare :Márk Gárdonyi; Teknisk mikrobiologi; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Biokemisk teknik; Biochemical technology; xylose isomerase; xylose transport; xylose fermentation; Saccharomyces cerevisiae; metabolic engineering;

    Sammanfattning : The aim of the thesis is to improve the initial steps of xylose metabolism in recombinant Saccharomyces cerevisiae. S. cerevisiae takes up xylose of poor affinity by means of hexose transporters. Metabolic control analysis was used to investigate whether the low xylose utilisation rate is due to inefficient transport. LÄS MER

  4. 3. Biocatalytic transamination with recombinant Saccharomyces cerevisiae: Challenges and opportunities

    Författare :Nora Weber; Teknisk mikrobiologi; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Saccharomyces cerevisiae; transamination; whole-cell biocatalysis; pyruvate; PLP; thiamine; reduction;

    Sammanfattning : Chiral building blocks are important molecules for synthesis of fine chemicals and pharmaceuticals. Biocatalysis has gained in relevance over traditional organic methods for synthesis of chiral compounds, as reactions can be performed at high enantio-selectivity and purity with environmentally friendly, simple, and cheap methods. LÄS MER

  5. 4. Engineering xylose and arabinose metabolism in recombinant Saccharomyces cerevisiae

    Författare :Kaisa Karhumaa; Teknisk mikrobiologi; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; virologi; bakteriologi; mycology; Mikrobiologi; bacteriology; Saccharomyces cerevisiae ; arabinose; xylose; fermentation; lignocellulose; yeast; mykologi; Biochemical technology; Biokemisk teknik; virology; Microbiology; ethanol;

    Sammanfattning : Utilization of all sugars in lignocellulose hydrolysates is a prerequisite for economically feasible bioethanol production. The yeast commonly used for industrial ethanol production, Saccharomyces cerevisiae, is naturally unable to utilize pentose sugars xylose and arabinose, which constitute a large fraction of many lignocellulosic materials. LÄS MER

  7. 5. Physiological Engineering of Xylose Utilisation by Recombinant Saccharomyces cerevisiae

    Författare :Nina Quednau Meinander; Teknisk mikrobiologi; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; protein burden; physiology; fed-batch fermentation; chemostat; recombinant Saccharomyces cerevisiae; ethanol; xylose; xylitol; Microbiology; bacteriology; virology; mycology; Mikrobiologi; bakteriologi; virologi; mykologi;

    Sammanfattning : Xylitol production by recombinant, XYL1-expressing Saccharomyces cerevisiae was investigated in fed-batch fermentation using different cosubstrates for growth, and generation of reduced cofactors and maintenance energy. Xylose was converted into xylitol with 1:1 yield. LÄS MER