Sökning: "real-time polymerase chain reaction RT-PCR"

Visar resultat 1 - 5 av 12 avhandlingar innehållade orden real-time polymerase chain reaction RT-PCR.

  1. 1. Monitoring anti-infectives and antibiotic resistance genes : with focus on analytical method development, effects of antibiotics and national perspectives

    Författare :Ghazanfar Ali Khan; Jerker Fick; Klaus Kümmerer; Umeå universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; method development; on-line solid phase extraction liquid chromatography mass spectrometry SPE LC-MS MS ; antibiotics; anti-infectives; constructed wetlands CWs ; denatured gradient gel electrophoresis DGGE ; percentage removal efficiency PRE ; shannon index; antibiotic resistance genes ARGs ; real-time polymerase chain reaction RT-PCR ; drug formulation facilities DFF ; wastewater; epidemiology; pandemic; influenza; compliance;

    Sammanfattning : Antibiotics are biologically active and are globally used in humans and animal medicine for treatment and in sub-therapeutic amounts as growth promoters in animal husbandry, aquaculture and agriculture. After excretion, inappropriate disposal and discharge from drug production facilities they enter into water bodies either as intact drugs, metabolites or transformed products. LÄS MER

  3. 2. Standardization and application of quantitative PCR methods in patients with hematological malignancies

    Författare :Maria Malec; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :Polymerase chain reaction; RQ-PCR; Minimal Residual Disease; Acute leukemia; Hodgkin s Lymphoma; cytokines;

    Sammanfattning : Using modem combination chemotherapy regimens, it has been possible to increase the rate of complete remission (CR) in acute lymphoblastic (ALL) and acute myeloid leukemia (AML). The major reason for treatment failure in acute leukemia (AL) is the resistance of leukemic blasts to chemotherapeutic agents, which is accompanied by an inability of the immune system to eradicate residual leukemic cells surviving initial treatment. LÄS MER

  4. 3. Expression profiling using manifold supports

    Författare :Anette Hagberg; Joakim Lundeberg; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Genetics; mRNA; expression; minimal residual disease MRD ; chronic myeloid leukemia CML ; recombinant human erythropoietin r-HuEpo ; anemia; insulin dependent diabetes mellitus IDDM ; transplantation; Genetik; Clinical genetics; Klinisk genetik; Medical Genetics; medicinsk genetik;

    Sammanfattning : Analyses of mRNA provides a condensed view of gene structure and quantitative analysis can reveal the induction of physiological or pathological gene expression programs. This thesis describes a new method for mRNA isolation, followed by sensitive real time detection via polymerase chain reaction (PCR), in order to quantitate transcripts of interest. LÄS MER

  5. 4. Aspects of inflammation and nitric oxide in cluster headache

    Författare :Anna Steinberg; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :Cluster Headache;

    Sammanfattning : Cluster Headache (CH) is an uncommon headache disorder, with severe implications for the individual patient. The headache is excruciating, unilateral and appearing in attacks. It is common that CH patients show ipsilateral associated symptoms, like for example conjunctival injection, lacrimation and nasal congestion. LÄS MER

  7. 5. PLASMA PROTEINS IN NORMAL AND PREECLAMPTIC PREGNANCY

    Författare :Karl Kristensen; Lund Obstetrik och gynekologi; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; gynaecology; andrology; reproduction; sexuality; Obstetrik; Obstetrics; pregnancy; renal function; serum amyloid A protein; Clinical chemistry; Klinisk kemi; preeclampsia; beta-2 microglobulin; C-reactive protein; cystatin C; inflammation; placenta; beta trace protein; gynekologi; andrologi; reproduktion; sexualitet;

    Sammanfattning : The aim of the research presented in this thesis was to provide extended background knowledge concerning several biochemical markers, used for the medical evaluation of pregnant women with suspected or confirmed preeclampsia, in order to improve the reliability of monitoring. Plasma levels of beta-2 microglobulin, cystatin C and beta trace protein are known to reflect renal filtration in non-pregnant settings, and the plasma proteins, C-reactive protein and serum amyloid A protein, are known to be sensitive markers of inflammation. LÄS MER