Sökning: "quantitative gene expression analysis"

Visar resultat 1 - 5 av 123 avhandlingar innehållade orden quantitative gene expression analysis.

1. 1. Gene Expression in Cancer Cells : Detection of Splice Variants, Allele-specific Expression and DNA Methylation

   Författare :Lili Milani; Ann-Christine Syvänen; Thomas R. Gingeras; Uppsala universitet; []
   Nyckelord :;

   Sammanfattning : The human genome sequencing project has provided a wealth of information on sequence variation between individuals. The surprisingly low number of genes in the human genome is compensated for by a complex regulation of gene expression. LÄS MER

3. 2. Quantification of gene expression in single cells

   Författare :Martin Bengtsson; Islet cell physiology; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Medicin människa och djur ; Medicine human and vertebrates ; Biomedical sciences; Biomedicinska vetenskaper; quantitative gene expression analysis; single cell PCR; islets of Langerhans;

   Sammanfattning : Studies of apparently homogeneous cell populations and single cells often give highly divergent results. Cells exhibit varying responsiveness to stimuli and gene expression and they are in many aspects stochastic and unpredictable. We have developed a method to measure gene expression quantitatively in individual cells with real-time RT-PCR. LÄS MER

4. 3. Gene Expression and DNA Methylation in Acute Lymphoblastic Leukemia

   Författare :Jessica Nordlund; Syvänen Ann-Christine; Xavier Estivill; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Digital gene expression; Allele-specific gene expression; DNA methylation; Epigenetics; Acute lymphoblastic leukemia; Molekylär medicin; Molecular Medicine;

   Sammanfattning : Pediatric acute lymphoblastic leukemia (ALL) is the most common malignancy in children, which results from the malignant transformation of progenitor cells in the bone marrow into leukemic cells. The precise mechanisms for this transformation are not well defined, however recent studies suggest that aberrant regulation of gene expression or DNA methylation may play an important role. LÄS MER

5. 4. In the quest for a cold tolerant variety : gene expression profile analysis of cold stressed oat and rice

   Författare :Angelica Lindlöf; Högskolan i Skövde; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; cold tolerance; oat; rice; bioinformatics analysis; Bioinformatics; Bioinformatik; Technology; Teknik; cold tolerance; oat; rice; bioinformatics analysis;

   Sammanfattning : Cold acclimation is a process which increases the freezing tolerance of an organism, after exposure to low, non-freezing temperatures. The acclimation ensures that cold tolerant species can endure harsh winter conditions, by preparing them to sub-zero temperatures. LÄS MER

7. 5. Expression profiling using manifold supports

   Författare :Anette Hagberg; Joakim Lundeberg; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Genetics; mRNA; expression; minimal residual disease MRD ; chronic myeloid leukemia CML ; recombinant human erythropoietin r-HuEpo ; anemia; insulin dependent diabetes mellitus IDDM ; transplantation; Genetik; Clinical genetics; Klinisk genetik; Medical Genetics; medicinsk genetik;

   Sammanfattning : Analyses of mRNA provides a condensed view of gene structure and quantitative analysis can reveal the induction of physiological or pathological gene expression programs. This thesis describes a new method for mRNA isolation, followed by sensitive real time detection via polymerase chain reaction (PCR), in order to quantitate transcripts of interest. LÄS MER