Sökning: "proteome profiling"

Visar resultat 1 - 5 av 61 avhandlingar innehållade orden proteome profiling.

1. 1. Profiling the cancer proteome

   Författare :Sofia Waldemarson; Institutionen för immunteknologi; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Cytologi; onkologi; cancer; Biotechnology; Bioteknik; cancerology; oncology; Cytology; iTRAQ; biomarkers; DIGE; two-dimensional gel electrophoresis; Proteomics;

   Sammanfattning : Cancer is a complex and heterogeneous disease where cells have started to grow uncontrolled and this disease remains a major health problem. Because of diffuse symptoms it often presents at a late stage. LÄS MER

3. 2. Mapping the human proteome using bioinformatic methods

   Författare :Linn Fagerberg; Mathias Uhlén; Alvis Brazma; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; proteome; transcriptome; bioinformatics; membrane protein prediction; subcellular localization; protein expression level; cell line; immunohistochemistry; immunofluorescence; Bioinformatics; Bioinformatik; SRA - Molecular Bioscience; SRA - Molekylär biovetenskap;

   Sammanfattning : The fundamental goal of proteomics is to gain an understanding of the expression and function of the proteome on the level of individual proteins, on the level of defined cell types and on the level of the entire organism. In this thesis, the human proteome is explored using membrane protein topology prediction methods to define the human membrane proteome and by global protein expression profiling, which relies on a complex study of the location and expression levels of proteins in tissues and cells. LÄS MER

4. 3. Understanding the Noise : Spliceosomal snRNA Profiling

   Författare :Lei Liu Conze; Anders Virtanen; Lars Wieslander; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; ncRNA; snRNA; U1; splice site; alternative splicing; high-throughput sequencing; 454; SNP; Molekylär cellbiologi; Molecular Cellbiology;

   Sammanfattning : The concept of the gene has been constantly challenged by new discoveries in the life sciences. Recent challenging observations include the high frequency of alternative splicing events and the common transcription of non-protein-coding-RNAs (ncRNAs) from the genome. The latter has long been considered noise in biological systems. LÄS MER

5. 4. Proteome profiling of human breast cancer

   Författare :Olena Zakharchenko; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :;

   Sammanfattning : Breast cancer (BC) takes thousands of woman’s lives yearly. Several factors have been found to influence initiation and development of breast cancer, and to affect prognosis and treatment of this disease. LÄS MER

7. 5. Global Proteome Survey -Transforming antibody-based affinity proteomics into a global discovery platform

   Författare :Niclas Olsson; Institutionen för immunteknologi; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; affinity proteomics; recombinant antibodies; Proteomics; mass spectrometry; proteome profiling; breast cancer; antibody specificity;

   Sammanfattning : Proteomics is expected to generate new insights into biological processes as well as identify novel biomarkers and therapeutic targets since most biological functions are transmitted through proteins. However, due to the complexity displayed by a proteome and inherent limitations associated with current methodologies, proteomic analyses often result in incomplete coverage and inconsistent measurements. LÄS MER