Sökning: "protein orientation"

Visar resultat 1 - 5 av 96 avhandlingar innehållade orden protein orientation.

1. 1. Molecular principles of protein stability and protein-protein interactions

   Författare :Christofer Lendel; Per-Åke Nygren; Christopher M. Dobson; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; affibody; binding thermodynamics; induced fit; molten globule; NMR spectroscopy; protein engineering; protein-protein interactions; protein stability; protein structure.; Structural biochemistry; Strukturbiokemi;

   Sammanfattning : Proteins with highly specific binding properties constitute the basis for many important applications in biotechnology and medicine. Immunoglobulins have so far been the obvious choice but recent advances in protein engineering have provided several novel constructs that indeed challenge antibodies. LÄS MER

3. 2. Structural and thermodynamical basis for molecular recognition between engineered binding proteins

   Författare :Jakob Dogan; Per-Åke Nygren; John E. Ladbury; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; protein structure; induced fit; binding thermodynamics; NMR spectroscopy; protein engineering; protein-protein interactions; protein stability; calorimetry; Structural biochemistry; Strukturbiokemi;

   Sammanfattning : The structural determination of interacting proteins, both as individual proteins and in their complex, complemented by thermodynamical studies are vital in order to gain in-depth insights of the phenomena leading to the highly selective protein-protein interactions characteristic of numerous life processes. This thesis describes an investigation of the structural and thermodynamical basis for molecular recognition in two different protein-protein complexes, formed between so-called affibody proteins and their respective targets. LÄS MER

4. 3. Cell-protein-material Interactions on Bioceramics and Model Surfaces

   Författare :Åsa Rosengren; Sven Oscarsson; Lucy Di Silvio; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Chemistry; protein adsorption; blood proteins; biomaterials; ceramics; glass-ceramics; two-dimensional gel electrophoresis; protein orientation; silicon wafers; atomic force microscopy; bone cell adhesion; divalent cation dependency; Kemi; Chemistry; Kemi;

   Sammanfattning : The objective of this thesis was to investigate and characterize the interaction between blood proteins and different surfaces with emphasis on protein adsorption to bioceramics and model surfaces. Special effort was made to monitor the spontaneous and selective adsorption of proteins from human plasma and to examine the orientation, conformation and functional behavior of single proteins after adsorption. LÄS MER

5. 4. Biosensor surface chemistry for oriented protein immobilization and biochip patterning

   Författare :Emma Ericsson; Karin Enander; Sofia Svedhem; Linköpings universitet; []
   Nyckelord :Biochip; biosensor; hydrogel; ligand; orientation; patterning; photochemistry; protein immobilization; surface chemistry; self-assembled monolayer; SAM;

   Sammanfattning : This licentiate thesis is focused on two methods for protein immobilization to biosensor surfaces for future applications in protein microarray formats. The common denominator is a surface chemistry based on a gold substrate with a self-assembled monolayer (SAM) of functionalized alkanethiolates. LÄS MER

7. 5. Interactions between Wood Polymers in Wood Cell Walls and Cellulose/Hemicellulose Biocomposites

   Författare :Jasna Stevanic Srndovic; Chalmers tekniska högskola; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; dynamic FTIR spectroscopy; viscoelasticity; orientation; Norway spruce; protein; glucomannan; xyloglucan; polymer interactions; pectin; imaging FTIR microscopy; primary cell wall; nanocomposite films; dynamic 2D FTIR spectroscopy; xylan; wood fibre; cellulose; secondary cell wall; DMA; lignin;

   Sammanfattning : A wood fibre is a complex multi component biocomposite that is hierarchically organised. The arrangement of a wood fibre on the ultrastructural level is highlycontrolled by the interactions between the main structural polymers, i.e. cellulose, various hemicelluloses and lignin, as well as in some parts also pectin and protein. LÄS MER