Sökning: "protein metabolism."

Visar resultat 1 - 5 av 872 avhandlingar innehållade orden protein metabolism..

  1. 1. Protein Folding Studied by Site Directed Mutagenesis

    Författare :Tomas Ternström; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Metabolism; Biokemi; metabolism; Biochemistry; Transition state; Activation Barrier; Two-State; Protein Folding; Protein Engineering;

    Sammanfattning : The N-terminal RNA binding domain of U1A has been shown to fold in a two-state process without accumulating any intermediates. U1A has a symmetrically curved chevron plot which seems to result mainly from changes of the transition state. LÄS MER

  3. 2. The conserved methionines in the chloroplast small heat shock protein - Role in chaperone-substrate interactions and effects of methionine sulfoxidation on chaperone activity

    Författare :Niklas Gustavsson; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Metabolism; Biochemistry; oxidative stress; thermostability; temperature stress; stress tolerance; sHsp; protein aggregation; molecular chaperone; Heat stress; methionine sulfoxidation; Biokemi; metabolism; Plant biochemistry; Växtbiokemi;

    Sammanfattning : The chloroplast-localized small heat shock protein (sHsp) Hsp21 belongs to the family of alpha-crystallin like sHsps which form large oligomeric structures and protect partly unfolded aggregation-prone proteins against aggregation. The main focus for this thesis has been on the highly conserved methionines in Hsp21. LÄS MER

  4. 3. Peptide-tagged proteins in aqueous two-phase systems

    Författare :Anna Nilsson; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Metabolism; Biokemi; metabolism; Biochemistry; green fluorescent protein; cutinase; tryptophan; Aqueous two-phase system; peptide-tagged protein;

    Sammanfattning : This thesis deals with proteins containing peptide tags for improved partitioning in aqueous two-phase systems. Qualitatively the peptide-tagged protein partitioning could be predicted from peptide data, i.e. partitioning trends found for peptides were also found for the peptide-tagged proteins. LÄS MER

  5. 4. Constraint-based modeling of yeast metabolism and protein secretion

    Författare :Feiran Li; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; protein secretion; metabolism; yeast evolution; genome-scale metabolic model; phenotype diversity;

    Sammanfattning : Yeasts are extensively exploited as cell factories for producing alcoholic beverages, biofuels, bio-pharmaceutical proteins, and other value-added chemicals. To improve the performance of yeast cell factories, it is necessary to understand their metabolism. LÄS MER

  7. 5. Cyclic Nucleotide Phosphodiesterase 3B: Regulation in rat adipocytes and 3T3-L1 cells

    Författare :Tova Landström; Institutionen för experimentell medicinsk vetenskap; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; 3T3-L1 TNF-alpha; adipocyte; insulin; p70S6 kinase; mitogen-activated protein kinase; protein kinase B; phosphatidylinositol-3 kinase; phosphodiesterase 3B; cAMP; lipolysis; phosphorylation; signal transduction; Biochemistry; Metabolism; Biokemi; metabolism;

    Sammanfattning : Insulin stimulation of rat adipocytes results in phosphorylation and activation of the cyclic nucleotide phosphodiesterase 3B (PDE3B), a key enzyme in the antilipolytic signalling pathway of this hormone. In this thesis, the site phosphorylated in PDE3B upon stimulation of rat adipocytes with insulin and/or the beta-adrenergic agonist, isoproterenol is identified. LÄS MER