Sökning: "probes"
Visar resultat 1 - 5 av 465 avhandlingar innehållade ordet probes.
1. Padlock probes : Circularizing oligonucleotides for localized detection of DNA sequence variants
Sammanfattning : Present techniques for localized detection of DNA sequence variants have limitations due to problems with sensitivity, specificity, signal localization or ability to perform many analyses simultaneously.In this study a novel gene analytic reagent is presented - the padlock probe. LÄS MER
2. Probes, pronouns and binding in the Minimalist Program
Sammanfattning : In chapter 2, I claim that not only heads, but phrases, too, are probes. I show that there are in fact no theoretical and empirical problems involved if we abandon the stipulation that only heads may probe. In chapter 3, I explore the structure of nominal expressions. LÄS MER
3. Padlock Probes and Rolling Circle Amplification : New Possibilities for Sensitive Gene Detection
Sammanfattning : A series of novel methods for detection of known sequence variants in DNA, in particular single nucleotide polymorphism, using padlock probes and rolling circle replication are presented. DNA probes that can be circularized – padlock probes – are ideal for rolling circle replication. LÄS MER
4. Nuclease Activity as a Biomarker in Cancer Detection
Sammanfattning : Nucleases are a group of enzymes that cleave the phosphodiester bonds in nucleic acids. As such, nucleases act as biological scissors that exhibit a plethora of fundamental roles, in prokaryotes and eukaryotes, dependent or non-dependent on their catalytic capability. LÄS MER
5. Fluorescent Probes and Protein Misfolding: Methods and Applications
Sammanfattning : Protein misfolding is a frequently occurring event in living cells and without the quality control, the consequences are disasterous. In this thesis, we studied protein misfolding in vitro by employing a variety of fluorophores, to either detect the highly ordered aggregates of intrinsically disordered proteins/peptides or slight misfolding of a natively folded protein. LÄS MER