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Visar resultat 1 - 5 av 55 avhandlingar som matchar ovanstående sökkriterier.

1. 1. Systems enabling antibody-mediated proteomics research

   Författare :Ronny Falk; Sophia Hober; Christer Wingren; KTH; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Antibody generation; dual expression; affinity purification; E. coli expression; Affibody molecules; polyclonal antibody; monospecific antibody; Immunobiology; Immunbiologi;

   Sammanfattning : As many genome sequencing efforts today are completed, we are now provided with the genetic maps for several organisms, including man. With these maps at hand, the scientific focus is now shifting towards investigations of the functionality of proteins. LÄS MER

3. 2. Production of polyclonal antibodies in rabbits and chickens immunised with human immunoglobulin G : A study of the utility of egg yolk antibody production as a substitute for rabbit serum antibody production

   Författare :Lise Svendsen Bollen; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Laboratory animals; antibody production; egg yolk; adjuvants; antibody response; avidity; Försöksdjursvetenskap; Laboratory animal science; Försöksdjursvetenskap; zoologisk utvecklingsbiologi; Animal Development;

   Sammanfattning : The utility of chicken egg yolk antibodies as an alternative to rabbit antisera was studied by comparing antibody and avidity development in immunised animals (15 rabbits and 30 chickens) during a one-year immunisation scheme. Human IgG was used as the model antigen and the efficacy of three adjuvants, Freund's Complete Adjuvant, Freund's Incomplete Adjuvant and Hunter's TiterMax, was compared. LÄS MER

4. 3. Characterization of antibody specificity using peptide array technologies

   Författare :Björn Forsström; Mathias Uhlén; Markus Templin; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Antibody; Epitope mapping; Peptide array; Suspension bead array; Antigen; Specificity; Cross-reactivity; Immunization; Immunogenicity; Bioteknologi; Biotechnology;

   Sammanfattning : Antibodies play an important role in the natural immune response to invading pathogens. The strong and specific binding to their antigens also make them indispensable tools for research, diagnostics and therapy. LÄS MER

5. 4. Epitope mapping of antibodies towards human protein targets

   Författare :Barbara Hjelm; Mathias Uhlén; Larry Gold; KTH; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; antibody; antibody validation; biomarker; epitope mapping; peptide array; proteomics; RBM3; staphylococcal surface display;

   Sammanfattning : This thesis, based on five research papers, presents results from development and evaluation ofmethods for identifying the interaction site of antibodies on their antigens and the functional investigation of these in different assays. As antibodies have proven to be invaluable tools in diagnostics, therapy and basic research, the demand of characterizing these binding molecules has increased. LÄS MER

7. 5. Utilizing Solid Phase Cloning, Surface Display And Epitope Information for Antibody Generation and Characterization

   Författare :Francis Jingxin Hu; Johan Rockberg; John McCafferty; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; antibody engineering; antibody affinity maturation; combinatorial protein engineering; epitope mapping; FACS; HER2; complement C5; Staphylococcal surface display; surface display.; Bioteknologi; Biotechnology;

   Sammanfattning : Antibodies have become indispensable tools in diagnostics, research and as therapeutics. There are several strategies to generate monoclonal antibodies (mAbs) in order to avoid the drawbacks of polyclonal antibodies (pAbs) for therapeutic use. LÄS MER