Sökning: "peptide mass fingerprinting"

Visar resultat 1 - 5 av 12 avhandlingar innehållade orden peptide mass fingerprinting.

  1. 1. Identification and Characterization of Peptides and Proteins using Fourier Transform Ion Cyclotron Resonance Mass Spectrometry

    Författare :Magnus Palmblad; Ole Nørregaard Jensen; Uppsala universitet; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Materials science; Peptide; protein; peptide mass fingerprinting; identification; sequencing; protein structure; non-covalent interaction; modification; electrospray ionization; liquid chromatography; capillary electrophoresis; electron capture dissociation; Fourier transform ion cyclotron resonance mass spectrometry; cerebrospinal fluid; amyloid β-peptide; Alzheimer’s disease.; Materialvetenskap; Materials science; Teknisk materialvetenskap; Molecular Biotechnology; Molekylär bioteknik;

    Sammanfattning : Mass spectrometry has in recent years been established as the standard method for protein identification and characterization in proteomics with excellent intrinsic sensitivity and specificity. Fourier transform ion cyclotron resonance is the mass spectrometric technique that provides the highest resolving power and mass accuracy, increasing the amount of information that can be obtained from complex samples. LÄS MER

  3. 2. Mass spectrometry in protein structure analysis

    Författare :Andreas Jonsson; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : Mass spectrometry is an important analytical tool in biological and biochemical research. The speed, accuracy and sensitivity is unmatched by conventional analytical techniques. LÄS MER

  4. 3. Sample preparation and mass spectrometry in proteome studies

    Författare :Daniel Hirschberg; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : Proteome studies aim to link gene to function. Instead of screening for function in a specific protein as in classical protein chemistry, proteins are now screened against a specific phenotype, disease or metabolic state. LÄS MER

  5. 4. Novel methods of tandem mass spectrometry in life sciences

    Författare :Mohammadhassan Gharibi; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : Mass spectrometry (MS) is a powerful analytical technique widely employed for isotopic analysis and species identification in numerous research fields. Isotopic analysis involves precisely measuring stable isotopes within a sample, offering valuable insights into its origin, environment, and biological processes. LÄS MER

  7. 5. Lipoproteomics : A New Approach to the Identification and Characterization of Proteins in LDL and HDL

    Författare :Helen Karlsson; Mats Lindahl; Christer Tagesson; Pia Davidsson; Linköpings universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; LDL; HDL; Proteomik; Atheroskleros; Masspektrometri; 2-DE; Molecular medicine; Molekylär medicin;

    Sammanfattning : A proteomic approach was applied to examine the protein composition of low-density lipoprotein (LDL) and high-density lipoprotein (HDL) in humans. LDL and HDL were isolated by density gradient ultracentrifugation, and proteins were separated with twodimensional gel electrophoresis (2-DE) and identified with peptide mass fingerprinting, using matrix-assisted laser desorption/ionization-time of flight mass spectrometry, and with amino acid sequencing using electrospray ionization tandem mass spectrometry. LÄS MER