Sökning: "peptide mapping"

Visar resultat 1 - 5 av 52 avhandlingar innehållade orden peptide mapping.

1. 1. Array-based Autoantibody Profiling and Epitope Mapping

   Författare :Arash Zandian; Peter Nilsson; Jochen M. Schwenk; Maja Neiman; Morten Nielsen; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; antibody; antigens; peptide; epitope mapping; autoimmunity; autoantibodies; microarrays; Bioteknologi; Biotechnology;

   Sammanfattning : Antibodies are a class of proteins that are made by the immune system to recognize harmful organisms and molecules. Their exceptional capability of specifically recognizing molecules has been investigated for over a century and information thereof has been utilized for a variety of applications including vaccine and generation of therapeutic antibodies. LÄS MER

3. 2. Characterization of antibody specificity using peptide array technologies

   Författare :Björn Forsström; Mathias Uhlén; Markus Templin; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Antibody; Epitope mapping; Peptide array; Suspension bead array; Antigen; Specificity; Cross-reactivity; Immunization; Immunogenicity; Bioteknologi; Biotechnology;

   Sammanfattning : Antibodies play an important role in the natural immune response to invading pathogens. The strong and specific binding to their antigens also make them indispensable tools for research, diagnostics and therapy. LÄS MER

4. 3. Epitope mapping of antibodies towards human protein targets

   Författare :Barbara Hjelm; Mathias Uhlén; Larry Gold; KTH; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; antibody; antibody validation; biomarker; epitope mapping; peptide array; proteomics; RBM3; staphylococcal surface display;

   Sammanfattning : This thesis, based on five research papers, presents results from development and evaluation ofmethods for identifying the interaction site of antibodies on their antigens and the functional investigation of these in different assays. As antibodies have proven to be invaluable tools in diagnostics, therapy and basic research, the demand of characterizing these binding molecules has increased. LÄS MER

5. 4. Managing and Exploring Large Data Sets Generated by Liquid Separation - Mass Spectrometry

   Författare :Daniel Bäckström; Per Sjöberg; Rolf Danielsson; Thomas Moritz; Uppsala universitet; []
   Nyckelord :Analytical chemistry; liquid chromatography; capillary electrophoresis; capillary electrochromatography; mass spectrometry; electro spray ionization; chemometrics; multivariate data analysis; peptide; biomarkers; fingerprints; PCA; alignment; data compression; ANOVA; target correlation; data management; Analytisk kemi;

   Sammanfattning : A trend in natural science and especially in analytical chemistry is the increasing need for analysis of a large number of complex samples with low analyte concentrations. Biological samples (urine, blood, plasma, cerebral spinal fluid, tissue etc. LÄS MER

7. 5. Liquid Chromatography Coupled to Mass Spectrometry : Implementation of Chemometric Optimization and Selected Applications

   Författare :My Moberg; Jonas Bergquist; Karin E. Markides; Dan Bylund; Douglas B. Kell; Uppsala universitet; []
   Nyckelord :Analytical chemistry; liquid chromatography; mass spectrometry; tandem mass spectrometry; electrospray ionization; inductively coupled plasma; chemometrics; optimization; multivariate data analysis; design of experiments; response surface model; siderophore; azathioprine; peptide; Analytisk kemi;

   Sammanfattning : Liquid chromatography (LC) coupled to mass spectrometry (MS) offers highly selective and sensitive analysis of a wide variety of compounds. However, the use of hyphenated experimental set-ups implies that many parameters may have an effect on the studied response. LÄS MER