Sökning: "paired antibodies"

Visar resultat 1 - 5 av 21 avhandlingar innehållade orden paired antibodies.

  1. 1. Validation of antibodies for protein profiling : A study using immunohistochemistry on tissue microarrays

    Författare :Linda Paavilainen; Fredrik Pontén; Ola Nilsson; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; antibody validation; monospecific antibodies; protein profiling; immunohistochemistry; tissue microarrays; western blot; fixatives; Pathology; Patologi; Pathology; patologi;

    Sammanfattning : The field of proteomics has rapidly expanded due to the completion of the human genome sequence. This thesis validates affinity-purified monospecific antibodies of polyclonal origin, for protein profiling in a broad spectrum of normal tissues and cells. LÄS MER

  3. 2. Automated Tissue Image Analysis Using Pattern Recognition

    Författare :Jimmy Azar; Anders Hast; Ewert Bengtsson; Martin Simonsson; Marco Loog; Uppsala universitet; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; tissue image analysis; pattern recognition; digital histopathology; immunohistochemistry; paired antibodies; histological stain evaluation; Computerized Image Processing; Datoriserad bildbehandling;

    Sammanfattning : Automated tissue image analysis aims to develop algorithms for a variety of histological applications. This has important implications in the diagnostic grading of cancer such as in breast and prostate tissue, as well as in the quantification of prognostic and predictive biomarkers that may help assess the risk of recurrence and the responsiveness of tumors to endocrine therapy. LÄS MER

  4. 3. Epitope mapping of antibodies towards human protein targets

    Författare :Barbara Hjelm; Mathias Uhlén; Larry Gold; KTH; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; antibody; antibody validation; biomarker; epitope mapping; peptide array; proteomics; RBM3; staphylococcal surface display;

    Sammanfattning : This thesis, based on five research papers, presents results from development and evaluation ofmethods for identifying the interaction site of antibodies on their antigens and the functional investigation of these in different assays. As antibodies have proven to be invaluable tools in diagnostics, therapy and basic research, the demand of characterizing these binding molecules has increased. LÄS MER

  5. 4. Barcoded DNA Sequencing for Parallel Protein Detection

    Författare :Mahya Dezfouli; Afshin Ahmadian; Ulf Landegren; KTH; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; DNA barcoding; antibody labeling; antibody oligonucleotide bio-conjugation; DNAassisted proteomics; immuno-sequencing I-Seq ; droplet-based system; large-scale data analysis; Biotechnology; Bioteknologi;

    Sammanfattning : The work presented in this thesis describes methodologies developed for integration and accurate interpretation of barcoded DNA, to empower large-scale-omics analysis. The objectives mainly aim at enabling multiplexed proteomic measurements in high-throughput format through DNA barcoding and massive parallel sequencing. LÄS MER

  7. 5. Chronic infection with chlamydia pneumoniae in COPD and lung cancer

    Författare :Eva Brandén; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :Chlamydia pneumoniae; chronic infection; lung cancer; COPD; treatment; detection;

    Sammanfattning : Lung cancer and chronic obstructive pulmonary disease (COPD) are major causes of morbidity and mortality throughout the world. Smoking is the main cause for both diseases, but other factors seem to contribute. LÄS MER