Sökning: "microchip"

Visar resultat 1 - 5 av 34 avhandlingar innehållade ordet microchip.

1. 1. True Monoliths as Separation Media : Homogeneous Gels for Electrophoresis and Electrochromatography in the Capillary and Microchip Modes

   Författare :Ákos Végvári; Douglas Westerlund; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; capillary electrophoresis; capillary electrochromatography; gel electrophoresis; agarose; polyacrylamide; b-cyclodextrin; DNA; peptide; glutathione; drug enantiomers; microchip; Biokemi; Biochemistry; Biokemi; biokemi; Biochemistry;

   Sammanfattning : The thesis focuses on the development of new homogeneous gels for the separation of drug enantiomers, peptides, DNA and virus by electrophoresis and electrochromatography in capillaries and microchips. This type of separation media offers high resolution and small zone broadening. LÄS MER

3. 2. Single Cell Investigations of the Functional Heterogeneity Within Immune Cell Populations : a Microchip-based Study

   Författare :Karolin Guldevall; Björn Önfelt; Hjalmar Brismar; Carsten Watzl; KTH; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Biologisk fysik; Biological Physics;

   Sammanfattning : Immune cell populations are constantly divided into smaller and smaller subsets defined by newly emerging cellular markers. However, there is a growing awareness of the functional heterogeneities in between cells even within small populations, in addition to the heterogeneity over time. LÄS MER

4. 3. Microscale Tools for Sample Preparation, Separation and Detection of Neuropeptides

   Författare :Andreas Dahlin; Jonas Bergquist; Karin E. Markides; Robert T. Kennedy; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Analytical chemistry; Neuropeptides; Microchip; Enhanced microdialysis; Poly dimethylsiloxane PDMS ; Electrospray ionization ESI ; Multidimensional separation; Electrochemical manipulation; Mass spectrometry MS ; Capillary electrophoresis CE ; Microdevice; Microfabrication; Micro total analysis system μ-TAS ; Analytisk kemi; Analytical chemistry; Analytisk kemi;

   Sammanfattning : The analysis of low abundant biological molecules is often challenging due to their chemical properties, low concentration and limited sample volumes. Neuropeptides are one group of molecules that fits these criteria. Neuropeptides also play an important role in biological functions, which makes them extra interesting to analyze. LÄS MER

5. 4. Analysis of proteins related toautoimmune and inflammatorydiseases with focus on newenrichment methodologies and MALDI-TOF MS

   Författare :Yuye Zhou; Åsa Emmer; Margret Thorsteinsdottir; KTH; []
   Nyckelord :Protein; Glycopeptide; IgG; Osteopontin; Enrichment; Digestion; Separation; Microchip; MALDI-TOF MS; Kemi; Chemistry;

   Sammanfattning : Proteins have been widely studied in biological sample analysis due to the association with diseases. In the present project, method and technique development was carried out on the analysis of two proteins, immunoglobulin G (IgG) and osteopontin (OPN). LÄS MER

7. 5. Electrochemical monitoring of living cells

   Författare :Christer Spegel; Centrum för analys och syntes; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; PC12; redox; electrochemistry; NAD P H; Single cell; Microchip; S. cerevisiae;

   Sammanfattning : This thesis presents the utilization of electroanalytical methods for analysis of dynamic cellular processes such as exocytosis, intracellular redox reactions and cell surface interactions. Microchips have been designed and manufactured with the purpose of monitoring dopamine exocytosis from single or a small ensemble of PC12 cells. LÄS MER