Sökning: "isoelectric focusing"

Visar resultat 1 - 5 av 21 avhandlingar innehållade orden isoelectric focusing.

  1. 1. Electrophoretic focusing in microchannels combined with mass spectrometry : Applications on amyloid beta peptides

    Författare :Saara Mikkonen; Åsa Emmer; Johan Jacksén; Tapio Kotiaho; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; amyloid beta; computer simulation; electrophoresis; electrospray ionization; isoelectric focusing; isotachophoresis; MALDI; mass spectrometry; microchannel; microchip; nano-electrospray ionization; preconcentration; separation; Kemi; Chemistry;

    Sammanfattning : Analysis of low-abundance components in small samples remains a challenge within bioanalytical chemistry, and new techniques for sample pretreatments followed by sensitive and informative detection are required. In this thesis, procedures for preconcentration and separation of proteins and peptides in open microchannels fabricated on silicon microchips are presented. LÄS MER

  3. 2. Analytical Approaches to Neurodegenerative Disease Protein Aggregation

    Författare :Henning Wiberg; Åsa Emmer; Torbjörn Gräslund; Leopold Ilag; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; neurodegenerative diseases; protein misfolding; protein aggregation; gel electrophoresis; isoelectric focusing; immunodetection; ELISA; immunoprecipitation; mass spectrometry; nanoelectrospray; liquid chromatography; neurodegenerativa sjukdomar; felveckade proteiner; proteinaggregering; geleektrofores; isoelektrisk fokusering; immundetektion; ELISA; immunoprecipitering; masspektrometri; nanoelektrospray; vätskekromatografi; Analytical chemistry; Analytisk kemi;

    Sammanfattning : .... LÄS MER

  4. 3. Isoelectric focusing of a transmembrane protein, the glucose transporter Glut1, and studies of detergent-Glut1 complexes

    Författare :Ann-Kristin Englund; Uppsala universitet; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP;

    Sammanfattning : .... LÄS MER

  5. 4. Proteomics of neurodegenerative diseases using novel online isoelectric point separation

    Författare :Mohammad Pirmoradian; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : The work presented in this thesis describes an instrument, developed for separation of proteins and peptides based on corresponding isoelectric point (pI) values, to empower mass spectrometry-based proteomics analysis. The main objectives are the instrument development and optimization, as well as clinical applications in biomarker discovery, particularly for neurodegenerative disorders. LÄS MER

  7. 5. Characterizing cancer cell signaling at the protein level : from targeted to proteome and phosphoproteome­‐wide analyses

    Författare :Elena Panizza; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : Proteins are the effectors of cellular functions and the constituting elements of cellular signaling cascades. The ability to analyze the abundances and the dynamics of proteins is central to dissect cellular signaling and its effects on cell physiology. LÄS MER