Sökning: "in-situ hybridization"

Visar resultat 6 - 10 av 295 avhandlingar innehållade orden in-situ hybridization.

1. 6. Improvements and Applications of in situ Proximity Ligation Assays

   Författare :Karin Grannas; Ola Söderberg; Christer Wingren; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Molekylär medicin; Molecular Medicine;

   Sammanfattning : The cells building up the human body is in constant communication with each other. This communication is done through large complex networks of signaling pathways for inter- and intracellular signal transduction. The signaling activity regulates many important processes, for example cell death, proliferation and differentiation. LÄS MER

3. 7. Detection and Sequencing of Amplified Single Molecules

   Författare :Rongqin Ke; Mats Nilsson; Ulf Landegren; Radoje Drmanac; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; padlock probes; proximity ligation assay; rolling circle amplification; circle-to-circle amplification; single molecule detection; amplified single molecules; sequencing; in situ; single cell; Molecular Biotechnology; Molekylär bioteknik;

   Sammanfattning : Improved analytical methods provide new opportunities for both biological research and medical applications. This thesis describes several novel molecular techniques for nucleic acid and protein analysis based on detection or sequencing of amplified single molecules (ASMs). LÄS MER

4. 8. Applications of in situ proximity ligation assays for cancer research and diagnostics

   Författare :Liza Löf; Masood Kamali-Moghaddam; Ulf Landegren; J.Paul Robinson; Uppsala universitet; []
   Nyckelord :Cancer; Diagnostics; Research; Prognostics; Method development; Flow cytometry; Drug delivery; Molekylär medicin; Molecular Medicine;

   Sammanfattning : In the field of cancer research and diagnostics it is crucial to have reliable methods for detecting molecules involved in the disease. New and better methods for diagnostics, prognostics and drug delivery therefore remain a permanent aim. LÄS MER

5. 9. Genotyping RNA and DNA using padlock probes

   Författare :Dan-Oscar Antson; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Genetics; Padlock probe; enzymatic synthesis; PCR; in situ hybridization; RNA-templated DNA ligation; Genetik; Clinical genetics; Klinisk genetik; Medical Genetics; medicinsk genetik;

   Sammanfattning : Novel techniques are needed to investigate the genetic variation revealed in the first draft of the human genome sequence. Padlock probes are recently developed reagents, suitable for detecting single-nucleotide variations of DNA and RNA in situ or in solution. LÄS MER

7. 10. Neuroprotection and neurogenesis after experimental stroke

   Författare :Andreas Arvidsson; Lund Neurologi; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; stereology; rat; Neurology; neuropsychology; neurophysiology; Neurologi; neuropsykologi; neurofysiologi; BrdU; immunohistochemistry; in situ hybridization; subventricular zone; striatum; dentate gyrus; neuronal replacement; neuronal survival; Focal cerebral ischemia; glial cell line-derived neurotrophic factor;

   Sammanfattning : In this thesis, two potential endogenous mechanisms for neuronal survival and repair have been investigated in a rat model of stroke, middle cerebral artery occlusion (MCAO): signaling of the GDNF family of neurotrophic factors, and neurogenesis from endogenous progenitor cells. As determined by in situ hybridization for GDNF family ligand and receptor genes, MCAO caused widespread upregulation of both components of the functional GDNF receptor, c-Ret and GFRalpha1, indicating that GDNF may be a survival signal in stroke. LÄS MER