Sökning: "homology-directed repair"

Visar resultat 1 - 5 av 6 avhandlingar innehållade orden homology-directed repair.

  1. 1. Cellular Origin and Development of Glioma

    Författare :Nanna Lindberg; Lene Uhrbom; Bengt Westermark; Kristian Pietras; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Glioma; brain tumor; PDGF; RCAS TV-A; cell of origin; glial cell; oligodendrocyte progenitor cell; DNA repair; homology-directed repair; RAD51; angiogenesis; histidine-rich glycoprotein; Morphology; cell biology; pathology; Morfologi; cellbiologi; patologi; Molecular Medicine; molekylär medicin genetik och patologi ;

    Sammanfattning : Gliomas are the most common primary tumors of the central nervous system believed to arise from glial cells. Invasive growth and inherent propensity for malignant progression make gliomas incurable despite extensive treatment. LÄS MER

  3. 2. DNA repair by HDR in experimental tumorigenesis

    Författare :Ulrica Westermark; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : The aim of this thesis was to further understand how defects in the homology-directed repair (HDR) pathway affect tumor formation and development. To study proteins involved in HDR is challenging since most of its members are required for cell viability. LÄS MER

  4. 3. Improving integration efficiency and precision of CRISPR/Cas9-mediated genome editing

    Författare :Sandra Wimberger; Göteborgs universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Genome editing; CRISPR-Cas9; DNA repair; genomic integration; off-targets;

    Sammanfattning : The clustered regularly interspaced short palindromic repeats (CRISPR) – CRISPR-associated protein 9 (Cas9) system has revolutionized the field of genome engineering, providing a cost-effective and fast tool for targeted gene modifications. Endogenous repair pathways, including error-prone non-homologous end joining and alternative end joining, or precise homology-directed repair (HDR), mend Cas9-induced DNA double-strand breaks. LÄS MER

  5. 4. Characterization of chromatin mobility upon DNA damage in Arabidopsis thaliana

    Författare :Anis Meschichi; Sveriges lantbruksuniversitet; Sveriges lantbruksuniversitet; []
    Nyckelord :LANTBRUKSVETENSKAPER; AGRICULTURAL SCIENCES;

    Sammanfattning : Plant cells are subject to high levels of DNA damage from dependence on sunlight for energy and the associated exposure to biotic and abiotic stresses. Double-strand breaks (DSBs) are a particularly deleterious type of DNA damage, potentially leading to chromosome rearrangements or loss of entire chromosome arms. LÄS MER

  7. 5. Identification of novel BRCA2-binding proteins that are essential for meiotic homologous recombination

    Författare :Jingjing Zhang; Göteborgs universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; meiosis; DSB; cancer; BRCA2; MEILB2; BRME1; HR; RAD51; DMC1; DDR;

    Sammanfattning : Meiotic recombination is a molecular process in which the induction and repair of programmed DNA double-strand breaks (DSBs) creates genetic exchange between homologous chromosomes and thus increases genetic diversity and ensures chromosome segregation. Breast cancer susceptibility gene 2 (BRCA2) is a potent cancer suppressor and is required for DSB repair by homologous recombination (HR) in mitosis. LÄS MER