Sökning: "fast determination"

Visar resultat 6 - 10 av 122 avhandlingar innehållade orden fast determination.

1. 6. Protein production, characterization and structure determination in structural genomics

   Författare :Esmeralda A. Woestenenk; Torleif Härd; Göran Karlsson; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biology; protein production; protein solubility; fusion tags; nuclear magnetic resonance; structure determination; Biologi; Biology; Biologi;

   Sammanfattning : This thesis covers the process from expression of a heterologous gene in Escherichia coli to structure determination of a protein by nuclear magnetic resonance (NMR) spectroscopy. The first part concerns structural genomics-related parallel screening studies on the effect of fusion tags (in particular the His tag) on protein solubility and the use of fusion tags in fast, parallel purification protocols intended for initial biophysical characterization of human proteins produced in E. LÄS MER

3. 7. A journey towards complete structure determination of zeolites by electron crystallography methods

   Författare :Elina Kapaca; Xiaodong Zou; Philippe Boullay; Stockholms universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Electron crystallography; Zeolites; Structure determination; Rotation electron diffraction; Continuous rotation electron diffraction; Disorder; Inorganic Chemistry; oorganisk kemi;

   Sammanfattning : Electron crystallography has recently become very successful for structural studies of materials with sub-micrometer sized crystals. In this thesis two major techniques have been applied for structure elucidation – 3-dimensional electron diffraction (3D ED) and high-resolution transmission electron microscopy (HRTEM) imaging. LÄS MER

4. 8. Dosimetry and radiation quality in fast-neutron radiation therapy : A study of radiation quality and basic dosimetric properties of fast-neutrons for external beam radiotherapy and problems associated with corrections of measured charged particle cross-sections

   Författare :Jonas Söderberg; Gudrun Alm Carlsson; Crister Ceberg; Linköpings universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Neutron; Dosimetry; Radiotherapy; Monte Carlo; Microdosimetry; Cross-section; RBE; LET; Energy-loss corrections; Radiological physics; Radiofysik;

   Sammanfattning : The dosimetric properties of fast-neutron beams with energies ≤80 MeV were explored using Monte Carlo techniques. Taking into account transport of all relevant types of released charged particles (electrons, protons, deuterons, tritons, 3He and α particles) pencil-beam dose distributions were derived and used to calculate absorbed dose distributions. LÄS MER

5. 9. New methods for determination of airborne pollutants : Focus on tetrabromobisphenol A, organophosphate triesters and polycyclic aromatic hydrocarbons

   Författare :Johanna Tollbäck; Ulrika Nilsson; Peter Haglund; Stockholms universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Air sampling; sampling methods; indoor air; SPE; SPME; extraction; dynamic extraction; online coupling; SWE; subcritical water extraction; GC MS; LC MS; organophosphate triesters; PAH; tetrabromobisphenol A; Analytical chemistry; Analytisk kemi; Analytical Chemistry; analytisk kemi;

   Sammanfattning : The work presented in this thesis concerns the development and evaluation of new methods of sampling and analysis of organic pollutants in the indoor and outdoor environment. In Paper I, the development of a new method was reported for the determination of the brominated flame retardant tetrabromobisphenol A (TBBPA) in air using sampling with glass fiber filter and polyurethane foam (PUF), ultrasonic solvent extraction and liquid chromatography coupled to electrospray ionisation mass spectrometry (LC-ESI/MS). LÄS MER

7. 10. Prediction, Design and Determination of Protein Structures

   Författare :Sebastian Rämisch; Biokemi och Strukturbiologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; protein design; Rosetta; Fold-and-Dock; leucine rich repeat proteins; coiled-coils; molecular replacement; ab initio phasing; temperature stability; fluorescent proteins;

   Sammanfattning : The three-dimensional structure of protein is encoded in its amino acid sequence. Modern structure prediction algorithms make it possible to predict the structure of small proteins using sequence information alone. LÄS MER