Sökning: "duplex stability"

Visar resultat 1 - 5 av 33 avhandlingar innehållade orden duplex stability.

1. 1. Nucleic Acids as Drug Targets - Influence of Platinum Complexes on Duplex Stability and Binding Kinetics

   Författare :Christopher Polonyi; Biokemi och Strukturbiologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Anticancer drugs; platinum complexes; cisplatin; oxaliplatin; nucleic acid; RNA; DNA; duplex melting; thermodynamic stability; kinetics; non-coding RNA; miRNA; siRNA; wobble base pair; loop; BRCA1; mimic; luciferase assay;

   Sammanfattning : Gaining a detailed understanding of how human cells are affected by metal-based drugs is crucial, since these drugs have shown great potential in cancer treatment. Cisplatin is used to treat many different types of cancer, e.g. testicular, ovarian, melanoma, bladder, non-small cell lung cancer, small cell lung cancer, myelomas and lymphomas. LÄS MER

3. 2. Expanding the alphabet of fluorescent nucleic acid base analogues: Characterization of new members

   Författare :Anke Dierckx; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; fluorescent adenine analogue; fluorescent DNA base analogue; duplex stability; Fluorescence quantum yield; circular dichroism; B-DNA;

   Sammanfattning : Fluorescence has proven to be a powerful tool for studying structure, dynamics and interactions of biomolecules such as proteins, RNA and DNA. However, since the natural DNA/RNA nucleobases are virtually non-fluorescent, there is a need for developing fluorescent nucleic acid markers. LÄS MER

4. 3. Fluorescent Nucleobase Analogues and their use for Investigating DNA Interactions

   Författare :Anke Dierckx; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; FRET; B-DNA; circular dichroism; fluorescent nucleobase analogue; adenine analogue; duplex stability; Fluorescence quantum yield;

   Sammanfattning : Ever since unravelling the structure of DNA, an expanding research field has emerged with ongoing efforts dedicated to increase our understanding of the molecule of life. Since the natural nucleobases are virtually non-emissive, it has been a challenge for decades to ‘light up’ DNA/RNA in order to investigate their properties utilizing fluorescence techniques. LÄS MER

5. 4. Ab initio Investigation of Al-doped CrMnFeCoNi High-Entropy Alloys

   Författare :Xun Sun; Levente Vitos; Andreas Blomqvist; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; High-entropy alloys; Phase stability; Elastic anisotropy; Brittle ductile transition; Pugh criterion; First-principles calculation; Teknisk materialvetenskap; Materials Science and Engineering;

   Sammanfattning : High-entropy alloys (HEAs) represent a special group of solid solutions containing five or more principal elements. The new design strategy has attracted extensive attention from the materials science community. The design and development of HEAs with desired properties have become an important subject in materials science and technology. LÄS MER

7. 5. Conformationally Constrained Oligonucleotides for RNA Targeting

   Författare :Qing Li; Jyoti Chattopadhyaya; Li-He Zhang; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; conformationally constrained nucleoside; antisense oligonucleotide; RNA affinity; nuclease stability; RNase H; RNA degradation; Chemistry with specialization in Bioorganic Chemistry; Kemi med inriktning mot bioorganisk kemi;

   Sammanfattning : A short oligonucleotide sequence as in a single-stranded antisense oligo nucleotides (AON) or in double-stranded small interfering RNAs (siRNA) can modulate the gene expression by targeting against the cellular mRNA, which can be potentially exploited for therapeutic purposes in the treatment of different diseases. In order to improve the efficacy of oligonucleotide-based drugs, the problem of target affinity, nuclease stability and delivery needs to be addressed. LÄS MER