Sökning: "dual expression"

Visar resultat 1 - 5 av 156 avhandlingar innehållade orden dual expression.

  1. 1. Systems enabling antibody-mediated proteomics research

    Författare :Ronny Falk; Sophia Hober; Christer Wingren; KTH; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Antibody generation; dual expression; affinity purification; E. coli expression; Affibody molecules; polyclonal antibody; monospecific antibody; Immunobiology; Immunbiologi;

    Sammanfattning : As many genome sequencing efforts today are completed, we are now provided with the genetic maps for several organisms, including man. With these maps at hand, the scientific focus is now shifting towards investigations of the functionality of proteins. LÄS MER

  3. 2. Organization and regulation of mitochondrial gene expression

    Författare :Roger Salvatori; Martin Ott; Benoit Kornmann; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Mitochondria; mitochondrial gene expression; post-transcriptional regulation; mitochondrial ribosome; protein-protein proximity; BioID; feedback loop; Biochemistry; biokemi;

    Sammanfattning : Mitochondria possess their own genome, remnant of the ancestral eubacterial endosymbiont DNA. This mitochondrial genome encodes mostly few key subunits of the respiratory chain. In order to synthesize these few proteins, mitochondria contain a complete gene expression machinery. LÄS MER

  4. 3. Transcriptome and Proteome Analysis using Signature Tags

    Författare :Charlotta Agaton; KTH; []
    Nyckelord :functional genomics; 3´-end signature tags; pyrosequencing; amplification; PrEST; chromosome 21; polyclonal antibodies; dual expression; affinity purification;

    Sammanfattning : With the full sequence of the human genome now available, anexciting era in biomedical research has started. The sequenceprovides information about all our genes and greatly increasesthe scope to compare genetic activities in different cells, toanalyze genetic variation between individuals and betweendifferent species and, most importantly, to investigatesystematically the whole genome in a gene-by-gene manner, andthus increase our understanding of gene function. LÄS MER

  5. 4. Improved detection and performance of surface expression from the AIDA-I autotransporter

    Författare :Johan Jarmander; Gen Larsson; Ulrika Rova; Ines Ezcurra; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; surface expression; autotransport; AIDA-I; Escherichia coli; proteolysis; detection tag;

    Sammanfattning : Surface expression of recombinant proteins has attracted a lot of attention due to its potential in applications such as enzyme production, vaccine delivery and bioremediation. Autotransporters have been used for surface expression of a variety of proteins, but the expression systems reported in literature have typically been inflexible and incapable of detecting proteolysis, thereby limiting surface expression yield. LÄS MER

  7. 5. Gene Expression Profiling of Human Atherosclerosis

    Författare :Sara Hägg; Johan Björkegren; Jesper Tegnér; Josefin Skogsberg; Olle Melander; Linköpings universitet; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP;

    Sammanfattning : Atherosclerosis is a progressive inflammatory disease that causes lipid accumulation in the arterial wall, leading to the formation of plaques. The clinical manifestations of plaque rupture—stroke and myocardial infarction—are increasing worldwide and pose an enormous economic burden for society. LÄS MER