Sökning: "capillary gel electrophoresis"

Visar resultat 1 - 5 av 16 avhandlingar innehållade orden capillary gel electrophoresis.

  1. 1. True Monoliths as Separation Media : Homogeneous Gels for Electrophoresis and Electrochromatography in the Capillary and Microchip Modes

    Författare :Ákos Végvári; Douglas Westerlund; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; capillary electrophoresis; capillary electrochromatography; gel electrophoresis; agarose; polyacrylamide; b-cyclodextrin; DNA; peptide; glutathione; drug enantiomers; microchip; Biokemi; Biochemistry; Biokemi; biokemi; Biochemistry;

    Sammanfattning : The thesis focuses on the development of new homogeneous gels for the separation of drug enantiomers, peptides, DNA and virus by electrophoresis and electrochromatography in capillaries and microchips. This type of separation media offers high resolution and small zone broadening. LÄS MER

  3. 2. Electrochemical Methods for Drug Characterisation and Transdermal Delivery : Capillary Zone Electrophoresis, Conductometry, and Iontophoresis

    Författare :Nadia Merclin; Per Hansson; Sven Engström; Arto Urtti; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Pharmaceutical chemistry; Capillary zone electrophoresis; precision conductometry; iontophoresis; passive diffusion; lidocaine; ALA; ALA-ester derivatives; sponge phase; carbopol gel; Farmaceutisk kemi; Pharmaceutical chemistry; Farmaceutisk kemi;

    Sammanfattning : This thesis concerns the development and utilisation of techniques for characterisation and transdermal delivery of various systems for pharmaceutical applications.The degree of dissociation of drug molecules and the mobilities of the different species formed are essential factors affecting the rate of drug delivery by iontophoresis. LÄS MER

  4. 3. Column Development in Capillary Electrophoresis and Electrochromatography for Bioanalytical Applications

    Författare :Nina Johannesson; Jonas Bergquist; Karin Markides; Abdul Malik; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Analytical chemistry; Capillary electrophoresis CE ; Capillary electrochromatography CEC ; Mass spectrometry MS ; Bioanalysis; Sample pretreatment; Sol-gel; Analytisk kemi;

    Sammanfattning : Analysis of biological samples can be a difficult task. This thesis covers a broad aspect of the analytical areas of capillary electrophoresis (CE) and capillary electrochromatography (CEC) in combination with mass spectrometry (MS) that are of great importance for achieving fast, accurate and sensitive bioanalyses. LÄS MER

  5. 4. Electrifying the Molecules of Life : Peptide and Protein Analysis by Capillary Electrophoresis Coupled to Electrospray Ionization Mass Spectrometry

    Författare :Magnus Wetterhall; Jonas Bergquist; Karin E. Markides; Dominic M. Desiderio; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Analytical chemistry; Capillary electrophoresis CE ; Electrospray ionization ESI ; Mass spectrometry MS ; Peptides; Proteins; Cerebrospinal fluid CSF ; Graphite coating; Monomer coating; Polymer coating; Analytisk kemi; Analytical chemistry; Analytisk kemi;

    Sammanfattning : This thesis describes the current status and novel aspects of the analysis of the molecules of life, i.e. peptides and proteins, using capillary electrophoresis (CE) coupled to mass spectrometry (MS) via (sheathless) electrospray ionization (ESI). Early reports of sheathless CE-ESI-MS were plagued by limited lifetimes of the electrospray emitter. LÄS MER

  7. 5. Analytical Quality by Design Method Development for Vaccine Characterization

    Författare :Lars Geurink; Catharina Sänger van de Griend; Mikael Hedeland; Martijn Schenning; Iulia Oita; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; viral vaccines; adenovirus; influenza; polio; COVID; corona; capillary electrophoresis; analytical quality by design; analytical method development; CGE; icIEF; CZE; crude cell suspension analysis; in-process sample analysis; quality control testing; biopharmaceutical characterization; Analytisk farmaceutisk kemi; Analytical Pharmaceutical Chemistry;

    Sammanfattning : Vaccines that are safe, efficacious, and can be rapidly developed are needed to prevent and to react to emerging global infectious disease threats such as influenza, Polio, and Coronavirus diseases. Fast and reliable analytical methods are required without delay to support vaccine process and product development, characterization, and quality control testing. LÄS MER