Sökning: "beta-galactosidase"

Visar resultat 1 - 5 av 11 avhandlingar innehållade ordet beta-galactosidase.

1. 1. Effects of fusion tags on protein partitioning In aqueous two-phase systems and use in primary protein recovery

   Författare :Cynthia Hassinen; KTH; []
   Nyckelord :E. coli; Aqueous two-phase systems; Fusion proteins; Hydrophobic interaction chromatography; Expanded bed adsorption; Beta-galactosidase; Klenow polymerase; Z-domain; Peptide tags;

   Sammanfattning : The two techniques aqueoustwo-phase partitioning and expanded bed adsorption that bothare suitable for primary protein recovery were studied. Most ofthe work was focused on partition in aqueous two-phase systemsand in particular on the possibility to effect the partitionbehaviour by fusion of short peptide tags or protein domains tothe target protein. LÄS MER

3. 2. Dynamic Sulfur Chemistry : Screening, Evaluation and Catalysis

   Författare :Rémi Caraballo; Olof Ramström; Nicolas Giuseppone; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Dynamic combinatorial chemistry; dynamic sulfur chemistry; thioglycoside; mutarotation; disulfide metathesis; hemithioacetal formation; 1H STD-NMR; inhibition; phosphine; Concanavalin A; beta-galactosidase; dynamic drug discovery.; Chemistry; Kemi; Organic chemistry; Organisk kemi; Bioorganic chemistry; Bioorganisk kemi;

   Sammanfattning : This thesis deals with the design, formation and evaluation of dynamic systems constructed by means of sulfur-containing reversible reactions, in organic and aqueous media and under mild conditions. In a first part, the synthesis of thioglycoside derivatives, constituting the biologically relevant starting components of the dynamic systems, is described. LÄS MER

4. 3. Orientation and Conformation of Single Macromolecules on Unmodified and Functionalized Surfaces

   Författare :Magnus Bergkvist; David Cullen; Uppsala universitet; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Biotechnology; Atomic Force Microscopy; protein orientation; protein adsorption; surface derivatization; Bioteknik; Bioengineering; Bioteknik; Surface Biotechnology; ytbioteknik;

   Sammanfattning : In this thesis methods for investigation of orientation and conformation of individual macromolecules on surfaces are presented as well as novel methods for functionalization of silicon chips with the possibility to get an ordered immobilization of antibodies. Two novel methods are presented which makes it possible to investigate the orientation of individual macromolecules on different kinds of surfaces with AFM. LÄS MER

5. 4. Treatment of Skin Wounds with Cell Transplantation and Gene Transfer

   Författare :Tor Svensjö; Lunds universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; pigs; gene regulation; cell culture; fibroblasts; keratinocytes; wound healing; skin; gene transfer; Transplantation; gene therapy; Surgery; orthopaedics; traumatology; Kirurgi; ortopedi; traumatologi;

   Sammanfattning : Background: A large number of different wound coverings have been used in order to improve the wound microenvironment, and thus accelerate repair. Since almost two centuries, clinicians and researchers have developed techniques for skin transplantation. LÄS MER

7. 5. Mechanism behind the development of radiation-induced cardiovascular effects

   Författare :Venkata Ramesh Yentrapalli; Siamak Haghdoost; Andrzej Wojcik; Stockholms universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; molekylärgenetik; Molecular Genetics;

   Sammanfattning : Epidemiological studies on acutely and chronically exposed individuals indicate that ionising radiation could be a risk factor for cardiovascular diseases. Experimental data regarding radiation-induced cardiovascular late effects are limited and biological mechanisms behind these late effects are still unclear. LÄS MER