Sökning: "antisense transcription"

Visar resultat 1 - 5 av 34 avhandlingar innehållade orden antisense transcription.

  1. 1. Functional characterization of the small antisense RNA MicA in Escherichia coli

    Författare :Klas Ifeanyi Udekwu; Gerhart Wagner; Tony Romeo; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; small RNA; antisense; outer membrane; Hfq-dependence; RNase III; LuxS; Quorum sensing; Microbiology; Mikrobiologi; mikrobiologi; Microbiology;

    Sammanfattning : The Escherichia coli small RNA (sRNA) MicA was identified recently in a genomewide search for sRNAs. It is encoded between the genes gshA and luxS in E. coli and its close relatives. The function of sRNAs in bacteria is generally believed to be in maintenance of homeostasis via stress-induced modulation of gene expression. LÄS MER

  3. 2. Making Sense of Antisense

    Författare :Johan Reimegård; Gerhart Wagner; Hanah Margalit; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; sRNA; miRNA; RNA; target prediction; SOLiD siRNA; E.coli; D.discoideum; Biology; Biologi; Bioinformatics; Bioinformatik; Biologi med inriktning mot mikrobiologi; Biology with specialization in Microbiology; Biology with specialization in Molecular Biology; Biologi med inriktning mot molekylärbiologi;

    Sammanfattning : RNA is a highly versatile molecule with functions that span from being a messenger in the transfer from DNA to protein, a catalytic molecule important for key processes in the cell to a regulator of gene expression. The post-genomic era and the use of new techniques to sequence RNAs have dramatically increased the number of regulatory RNAs during the last decade. LÄS MER

  4. 3. Macromolecular Matchmaking : Mechanisms and Biology of Bacterial Small RNAs

    Författare :Erik Holmqvist; E. Gerhart H. Wagner; Udo Bläsi; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Small RNA; sRNA; non-coding RNA; antisense; gene regulation; post-transcriptional regulation; translational control; outer membrane protein; OmpA; MicA; biofilm; flagella; curli; bistability; Lrp; MicF; CsgD; FlgM; OmrA; OmrB; Biologi med inriktning mot mikrobiologi; Biology with specialization in Microbiology;

    Sammanfattning : Cells sense the properties of the surrounding environment and convert this information into changes in gene expression. Bacteria are, in contrast to many multi-cellular eukaryotes, remarkable in their ability to cope with rapid environmental changes and to endure harsh and extreme milieus. LÄS MER

  5. 4. Small RNA-mediated Regulation of Gene Expression in Escherichia coli

    Författare :Cecilia Unoson; E. Gerhart. H Wagner; Renée Schroeder; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Small RNA; non-coding RNA; antisense; translational control; ribosome standby; toxin-antitoxin; IstR; TisB; tisAB; MicF; Lrp; Microbiology; Mikrobiologi; Cell and molecular biology; Cell- och molekylärbiologi; Biologi med inriktning mot mikrobiologi; Biology with specialization in Microbiology;

    Sammanfattning : Non-coding RNAs are highly abundant regulators of gene expression in all kingdoms of life that often play important roles in vital cellular functions. In bacteria, small regulatory RNAs (sRNAs) usually act post-transcriptionally by regulating mRNAs through base pairing within ribosome binding sites (RBS), thereby inhibiting translation initiation. LÄS MER

  7. 5. Interactions of the Cell-Penetrating Peptide Penetratin with Model Membranes and Live Cells

    Författare :Daniel Persson; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; antisense; antennapedia homeodomain; delivery vector; cell-penetrating peptide; protein transduction; uptake; membrane translocation; penetratin; confocal microscopy; vesicle;

    Sammanfattning : The delivery of large, hydrophilic molecules, such as proteins and oligonucleotides, to the cytoplasm and nucleus of cells is problematic due to their generally low plasma membrane permeability. With the identification of a number of cell-penetrating peptides, capable of mediating non-endocytotic, intracellular delivery of macromolecular cargos, this obstacle now seems surmountable. LÄS MER